CLONING AND DEVELOPMENTAL EXPRESSION OF LFB3/HNF1-BETA TRANSCRIPTION FACTOR IN XENOPUS-LAEVIS
CLONING AND DEVELOPMENTAL EXPRESSION OF LFB3/HNF1-BETA TRANSCRIPTION FACTOR IN XENOPUS-LAEVIS
复制标题
DOI：
10.1016/0925-4773(94)90092-2
复制
发表时间：
1994-07-01
影响因子：
2.6
通讯作者：
DESIMONE, V
中科院分区：
文献类型：
--
作者：
DEMARTIS, A;MAFFEI, M;DESIMONE, V
我们克隆了非洲爪蟾LFB 3/HNF 1 β转录因子的同源物。RNA酶保护和原位杂交实验表明，XLFB 3转录开始于原肠胚内胚层在第10.5阶段（中期原肠胚）。在后期阶段，内胚层内的XLFB 3转录物限于中肠和后肠及其衍生器官和组织。XLFB 3也在神经外胚层和原肾原基中表达。XLFB 3在所有三个胚层的吻侧部分中不表达，具有通过用视黄酸处理发育中的胚胎而向前移动的一致的前边界。XLFB 3是早期内胚层分化的有用标志物，其沿前后轴的沿着表达模式以及对视黄酸处理的反应表明在早期形态发生中的作用。
We have cloned the Xenopus laevis homologue of the LFB3/HNF1 beta transcription factor. RNase protection and in situ hybridisation experiments show that XLFB3 transcription starts in the gastrulating endoderm at stage 10.5 (mid-gastrula). At later stages, XLFB3 transcripts within the endoderm are restricted to mid- and hindgut and to their derivative organs and tissues. XLFB3 is also expressed in the neuroectoderm and in the pronephros anlage. XLFB3 is not expressed in the rostral part of all three germ layers, with coincident anterior borders that are shifted anteriorly by treatment of developing embryos with retinoic acid. XLFB3 is a useful marker of early endoderm differentiation and its expression pattern along the antero-posterior axis, as well as the response to retinoic acid treatment, suggests a role in early morphogenesis.