PHYTOCHROME SIGNAL-TRANSDUCTION - CHARACTERIZATION OF PATHWAYS AND ISOLATION OF MUTANTS
PHYTOCHROME SIGNAL-TRANSDUCTION - CHARACTERIZATION OF PATHWAYS AND ISOLATION OF MUTANTS
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DOI：
10.1098/rstb.1995.0139
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发表时间：
1995-10-30
影响因子：
6.3
通讯作者：
CHUA, NH
中科院分区：
文献类型：
--
作者：
BARNES, SA;QUAGGIO, RB;CHUA, NH
光敏色素信号传导的研究已经产生了丰富的数据，描述了受体对光的感知，以及光敏色素通过许多转录因子调节基因表达的最终步骤。我们现在专注于建立将光敏色素光激活与基因表达联系起来的干预步骤，以及这些信号通路的调节和相互作用。最近的研究利用光养大豆悬浮培养的药理学方法和番茄的显微注射技术建立了三种不同的光敏色素信号转导途径：(i)钙依赖性途径，调节编码叶绿素a/b结合蛋白（CAB）和光系统II的其他成分的基因的表达；（ii） cgmp依赖性通路，调节编码查尔酮合成酶（CHS）的基因表达和花青素色素的产生；（iii）依赖钙和cGMP的途径，调节编码光系统I组分的基因表达，并且是成熟叶绿体产生所必需的。为了研究光敏色素信号转导途径的组成和调控，许多实验室已经分离出具有改变光形态形成反应的突变体。然而，除了一些可能的例外，光敏色素信号转导途径正调控元件突变体的分离几乎没有取得真正的进展。我们已经在拟南芥幼苗中描述了一种新的光敏色素a （phyA）介导的远红光（FR）响应，我们目前正在使用它来筛选特定的phyA信号转导突变体。
The study of phytochrome signalling has yielded a wealth of data describing both the perception of light by the receptor, and the terminal steps in phytochrome-regulated gene expression by a number of transcription factors. We are now focusing on establishing the intervening steps linking phytochrome photoactivation to gene expression, and the regulation and interactions of these signalling pathways. Recent work has utilized both a pharmacological approach in phototrophic soybean suspension cultures and microinjection techniques in tomato to establish three distinct phytochrome signal-transduction pathways: (i) a calcium-dependent pathway that regulates the expression of genes encoding the chlorophyll a/b binding protein (CAB) and other components of photosystem II; (ii) a cGMP-dependent pathway that regulates the expression of the gene encoding chalcone synthase (CHS) and the production of anthocyanin pigments; and (iii) a pathway dependent upon both calcium and cGMP that regulates the expression of genes encoding components of photosystem I and is necessary for the production of mature chloroplasts. To study the components and the regulation of phytochrome signal-transduction pathways, mutants with altered photomorphogenic responses have been isolated by a number of laboratories. However, with several possible exceptions, Little real progress has been made towards the isolation of mutants in positive regulatory elements of the phytochrome signal-transduction pathway. We have characterized a novel phytochrome A (phyA)-mediated far-red light (FR) response in Arabidopsis seedlings which we are currently using to screen for specific phyA signal-transduction mutants.