Architecture of the osteocyte network correlates with bone material quality
Architecture of the osteocyte network correlates with bone material quality
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DOI：
10.1002/jbmr.1927
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发表时间：
2013-08-01
影响因子：
6.2
通讯作者：
Fratzl, Peter
中科院分区：
文献类型：
--
作者：
Kerschnitzki, Michael;Kollmannsberger, Philip;Fratzl, Peter
在骨等生物组织中，细胞的功能和活动关键取决于细胞合成和条件下的细胞外基质的物理性质。在骨形成和重塑过程中，成骨细胞嵌入到它们沉积的基质中，并分化为骨细胞。这些细胞在整个骨材料中形成致密的网络。众所周知，骨细胞可以协调骨重建。然而，骨细胞在矿物质动态平衡中的确切作用及其对骨材料质量的潜在影响仍不清楚。为了了解骨细胞和细胞外基质的相互影响，揭示它们的网络组织与其周围材料的性质是至关重要的。在这里，我们使用共聚焦激光扫描显微镜对矿化骨切片中的骨细胞网络进行可视化和拓扑量化。在样品的同一区域，使用同步加速器小角X射线散射来确定纳米级骨矿物颗粒的大小和相对于细胞网络的排列。主要的发现是，大多数矿物颗粒位于距离最近的细胞网络通道不到一微米的范围内，并且矿物颗粒的特性取决于离细胞网络的距离。该网络的架构揭示了细胞之间和血管之间的运输成本的最优化。综上所述，这些发现定量地表明，由于骨细胞网络的致密组织，它提供了进入骨骼中巨大矿物质储存库的途径。观察到的骨细胞网络结构与骨材料特性之间的相关性支持了骨细胞与其矿化区域相互作用从而参与骨矿物质动态平衡的假设。(C)2013年美国骨与矿物研究学会。
In biological tissues such as bone, cell function and activity crucially depend on the physical properties of the extracellular matrix which the cells synthesize and condition. During bone formation and remodeling, osteoblasts get embedded into the matrix they deposit and differentiate to osteocytes. These cells form a dense network throughout the entire bone material. Osteocytes are known to orchestrate bone remodeling. However, the precise role of osteocytes during mineral homeostasis and their potential influence on bone material quality remains unclear. To understand the mutual influence of osteocytes and extracellular matrix, it is crucial to reveal their network organization in relation to the properties of their surrounding material. Here we visualize and topologically quantify the osteocyte network in mineralized bone sections with confocal laser scanning microscopy. At the same region of the sample, synchrotron small-angle X-ray scattering is used to determine nanoscopic bone mineral particle size and arrangement relative to the cell network. Major findings are that most of the mineral particles reside within less than a micrometer from the nearest cell network channel and that mineral particle characteristics depend on the distance from the cell network. The architecture of the network reveals optimization with respect to transport costs between cells and to blood vessels. In conclusion, these findings quantitatively show that the osteocyte network provides access to a huge mineral reservoir in bone due to its dense organization. The observed correlation between the architecture of osteocyte networks and bone material properties supports the hypothesis that osteocytes interact with their mineralized vicinity and thus, participate in bone mineral homeostasis. (C) 2013 American Society for Bone and Mineral Research.