Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles
Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles
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DOI：
10.1002/bit.20262
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发表时间：
2004-11-20
影响因子：
3.8
通讯作者：
Osaka, T
中科院分区：
文献类型：
--
作者：
Arakaki, A;Hideshima, S;Osaka, T
为了开发磁性生物测定系统，使用磁性纳米颗粒和具有自组装单层的硅基底进行了确定生物素和链霉亲和素之间存在特异性生物分子相互作用的研究。将链霉亲和素固定在磁性颗粒上，并将生物素附着到单层修饰的基底上。在光学显微镜下清楚地观察到链霉亲和素修饰的磁性颗粒在生物素修饰的基底上的反应。使用磁力显微镜检测来自颗粒的磁信号。这项研究的结果表明，单层改性的基板与生物分子改性的磁性颗粒的组合是有用的，用于检测生物分子的相互作用，在医疗和诊断分析。(C)2004 Wiley Periodicals，Inc.
For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses. (C) 2004 Wiley Periodicals, Inc.