Global translational induction during NLR-mediated immunity in plants is dynamically regulated by CDC123, an ATP-sensitive protein
Global translational induction during NLR-mediated immunity in plants is dynamically regulated by CDC123, an ATP-sensitive protein
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DOI：
10.1016/j.chom.2023.01.014
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发表时间：
2023-03-08
影响因子：
30.3
通讯作者：
Dong, Xinnian
中科院分区：
文献类型：
--
作者：
Chen, Tianyuan;Xu, Guoyong;Dong, Xinnian
病原体效应子通过其同源的核苷酸结合富含亮氨酸重复序列（NLR）受体识别激活植物中的效应子触发免疫（ETI）。ETI与相关的转录和翻译重编程以及随后的感染细胞死亡相关。ETI相关的翻译是主动调节还是被动驱动的转录动力学仍然是未知的。在使用翻译报告基因的遗传筛选中，我们鉴定了CDC 123，一种ATP-抓取蛋白，作为ETI相关翻译和防御的关键激活剂。在ETI期间，ATP浓度的增加促进了CDC 123介导的真核翻译起始因子2（eIF 2）复合物的组装。由于ATP是激活NLR以及CDC 123功能所必需的，我们发现了一种可能的机制，通过该机制，防御翻译组在NLR介导的免疫过程中被协同诱导。CDC 123介导的eIF 2 as-saccharide的保守性表明其在植物以外的NLR介导的免疫中可能起作用。
The recognition of pathogen effectors by their cognate nucleotide-binding leucine-rich repeat (NLR) recep-tors activates effector-triggered immunity (ETI) in plants. ETI is associated with correlated transcriptional and translational reprogramming and subsequent death of infected cells. Whether ETI-associated translation is actively regulated or passively driven by transcriptional dynamics remains unknown. In a genetic screen us-ing a translational reporter, we identified CDC123, an ATP-grasp protein, as a key activator of ETI-associated translation and defense. During ETI, an increase in ATP concentration facilitates CDC123-mediated assembly of the eukaryotic translation initiation factor 2 (eIF2) complex. Because ATP is required for the activation of NLRs as well as the CDC123 function, we uncovered a possible mechanism by which the defense translatome is coordinately induced during NLR-mediated immunity. The conservation of the CDC123-mediated eIF2 as-sembly suggests its possible role in NLR-mediated immunity beyond plants.