Quinolone resistance-determining region mutations and por type of Neisseria gonorrhoeae isolates:: Resistance surveillance and typing by molecular methodologies
Quinolone resistance-determining region mutations and por type of Neisseria gonorrhoeae isolates:: Resistance surveillance and typing by molecular methodologies
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DOI：
10.1086/386312
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发表时间：
2004-06-01
影响因子：
6.4
通讯作者：
Bash, MC
中科院分区：
文献类型：
--
作者：
Giles, JA;Falconio, J;Bash, MC
喹诺酮耐药性在淋病奈瑟菌中迅速增加，是一个重要的公共卫生问题，需要持续监测。探讨喹诺酮类药物耐药性分子监测的可行性，并进一步描述耐药N。80株淋病奈瑟氏球菌的喹诺酮类耐药决定区（QRDR）序列和por类型。使用分子技术确定淋病分离株。PCR扩增gyrA和parC的QRDR，直接测序。通过使用寡核苷酸探针对编码孔蛋白的5个可变环的区域进行棋盘杂交来确定por类型。所有42株环丙沙星耐药（CipR）分离株均在gyrA和parC的QRDR中存在突变，在这些分离株中有93%发现了相同的突变。1株中度耐药分离株gyrA有1个突变，敏感分离株无突变。40个分离株具有2种por类型中的1种，其差异仅在于可变区5中的框内缺失;除1种外，所有这些分离株均为CipR。QRDR序列测定和por分型结果表明，CipR N.以色列的淋病是克隆的。QRDR突变与以前的特征一致，这表明DNA探针可以开发用于快速检测和监测喹诺酮耐药的N。淋病在使用非培养诊断方法的环境中。
Quinolone resistance is increasing rapidly in Neisseria gonorrhoeae and is a significant public health problem that requires ongoing surveillance. To examine the feasibility of molecular surveillance of quinolone resistance, and to further characterize an outbreak of resistant N. gonorrhoeae in Israel, the quinolone resistance determining region (QRDR) sequences and the por types of 80 N. gonorrhoeae isolates were determined using molecular techniques. QRDRs of gyrA and parC were amplified by polymerase chain reaction and were sequenced directly. The por type was determined by checkerboard hybridizations performed using oligonucleotide probes to regions encoding 5 variable loops of the porin protein. All 42 ciprofloxacin-resistant (CipR) isolates had mutations in QRDRs of both gyrA and parC, and identical mutations were found in 93% of these isolates. One intermediately resistant isolate had 1 mutation in gyrA, and susceptible isolates showed no mutations. Forty isolates had 1 of 2 por types that differed only by an in-frame deletion in variable region 5; all but 1 of these isolates were CipR. QRDR sequencing and por type determination showed that the outbreak of CipR N. gonorrhoeae in Israel was clonal. QRDR mutations were consistent with those previously characterized; this indicates that DNA probes can be developed for rapid detection and surveillance of quinolone-resistant N. gonorrhoeae in settings in which nonculture diagnostic methods are used.