Role of nitric oxide in human esophageal circular smooth muscle in vitro.
Role of nitric oxide in human esophageal circular smooth muscle in vitro.
复制标题
一氧化氮在体外人食管环形平滑肌中的作用。
DOI：
10.1016/s0022-5223(05)80021-2
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Sugarbaker,DJ
中科院分区：
文献类型：
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作者：
Richards,WG;Stamler,JS;Kobzik,L;Sugarbaker,DJ
研究了一氧化氮在人食管平滑肌中的作用。对切除组织样本的纵向和圆形平滑肌层内的组成型一氧化氮合酶标记的神经纤维和神经束进行免疫染色。安装在器官浴中的圆形肌肉条表现出自发收缩和活跃的张力。当暴露于 20 Hz 的 5 秒电场刺激序列时，大多数条带表现出刺激内“开”和刺激后“关”收缩。暴露于 0.1 μmol/L（或更高）浓度的阿托品可将“开启”收缩转变为“开启”松弛，并使“关闭”收缩减少 63%。暴露于 NG-硝基-L-精氨酸会导致浓度依赖性的“开启”收缩增强和“关闭”收缩消除。过量的左旋精氨酸增强了这些效应的逆转。硝普钠抑制自发收缩和诱发收缩。这些结果表明，一氧化氮合成是人食管环形平滑肌神经抑制的介质，并且对于“关闭”收缩的发生是必需的。
The role of nitric oxide in human esophageal smooth muscle was examined. Immunostaining for constitutive nitric oxide synthase labeled nerve fibers and bundles within longitudinal and circular smooth muscle layers of resected tissue samples. Strips of circular muscle mounted in organ baths exhibited spontaneous contractions and active tone. When exposed to 5-second trains of electric field stimulation at 20 Hz, most strips exhibited intrastimulus “on” and poststimulus “off” contractions. Exposure to a 0.1 μmol/L (or greater) concentration of atropine converted “on” contractions to “on” relaxations and reduced “off” contractions by 63%. Exposure to NG-nitro-l-arginine resulted in concentration-dependent enhancement of “on” contractions and abolition of “off” contractions. Excess l-arginine enhanced the reversal of these effects. Sodium nitroprusside inhibited both spontaneous and evoked contractions. These results suggest that nitric oxide synthesis is a mediator of neural inhibition of human esophageal circular smooth muscle and is necessary for the occurrence of “off” contractions.