Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes.
Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes.
复制标题
人根素基因和两个分散的假基因的分子克隆、cDNA 序列和染色体分配。
DOI：
10.1006/geno.1993.1159
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Furthmayr,H
中科院分区：
文献类型：
--
作者：
Wilgenbus,KK;Milatovich,A;Francke,U;Furthmayr,H
Radixin是一种细胞骨架蛋白，可能在连接肌动蛋白和质膜中起重要作用。最近克隆的小鼠和猪radixin cDNA揭示了一种蛋白质高度同源埃兹蛋白和膜突蛋白。我们已经克隆和测序了人radixin的cDNA，发现预测的氨基酸序列的人蛋白质是几乎相同的预测radixin在其他两个物种。通过对中国仓鼠×人类体细胞杂种DNA及杂种PCR产物的Southern分析，将其编码基因（RDX）定位于11q。荧光染色体原位杂交与cDNA质粒进一步定位该基因带11q23。然而，在杂交DNA板上用“根蛋白特异性”引物进行PCR扩增产生了另外的非常相似的DNA序列，其进一步通过PCR产物的直接测序来表征。该序列代表截短的版本，相应的基因座（RDXP2）被分配给Xp21.3。此外，通过使用不同的引物组，发现第三个序列与radixin序列90%相同，但含有终止密码子，似乎缺乏内含子。通过Southern和PCR分析，将该假基因定位于11p。
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. We have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster × human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomalin situhybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with "radixin-specific" primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This sequence represents a truncated version and the respective locus (RDXP2) was assigned to Xp21.3. Furthermore, by employing a different set of primers, a third sequence was found that was 90% identical to the radixin sequence but contained termination codons and seemed to lack introns. This pseudogene (RDXP1) was mapped to 11p by Southern and PCR analyses.