A Tanshinone IIA Loaded Hybrid Nanocomposite with Enhanced Therapeutic Effect for Otitis Media.
A Tanshinone IIA Loaded Hybrid Nanocomposite with Enhanced Therapeutic Effect for Otitis Media.
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DOI：
10.1016/j.ijpharm.2019.118846
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发表时间：
2019-12
影响因子：
5.8
通讯作者：
Hang Yu;Pei Zeng;Yongshi Liang;Xiaozhu Chen;Haiyan Hu;Lu Wen;Gang Chen
中科院分区：
文献类型：
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作者：
Hang Yu;Pei Zeng;Yongshi Liang;Xiaozhu Chen;Haiyan Hu;Lu Wen;Gang Chen
中耳炎，通常称为中耳炎，是最常见的疾病之一，并导致显著的发病率，如听力损失。鉴于金黄色葡萄球菌等细菌的侵入是引起中耳炎的主要原因，药物治疗一般采用局部或全身抗菌的方法。然而，由于耐药性细菌菌株的迅速出现，抗菌剂的有效性正在减弱。本文设计并制备了一种银纳米粒子（AgNPs）基多组分杂化纳米复合材料TSIIA @ CS/Lys @ AgNPs，该复合材料由AgNPs核、壳聚糖（CS）或溶菌酶（Lys）中间层和丹参酮IIA（TSIIA）包合物外层组成。通过静电相互作用的CS或赖氨酸的AgNPs涂层可能产生类似核桃的内果皮的核壳纳米复合物。这种设计可以减少AgNPs的用量，同时保持抗菌活性，可能是由于纳米复合物和细菌之间的有利相互作用。通过包合络合法沉积中草药活性成分TSIIA形成杂化纳米复合材料的外层，朝着改善的抗菌性能，其显示出与临床上商业使用的注射给药的氧氟沙星相当的对豚鼠急性中耳炎的治疗效果。该杂化纳米复合材料以原位有机凝胶的形式分散于聚乳酸-羟基乙酸共聚物/N-甲基-2-吡咯烷酮（PLGA/NMP）溶液中，不仅保持了治疗效果，而且与溶液制剂相比具有注射频率低的优点。此外，杂化纳米复合材料和有机凝胶对健康豚鼠的基底膜和上皮组织无明显毒性作用。该研究为开发具有增强抗菌功效的杂化纳米复合材料提供了一种有前途的策略，也为建立有效的治疗系统开辟了一条新途径，减少了抗生素治疗中耳炎的给药频率。
Otitis media, commonly known as middle ear inflammation, is among one of the most common maladies and results in significant morbidity such as loss of hearing. In view of the bacteria invasion such as Staphylococcus aureus causes the majority forms of otitis media, drug treatment generally uses antibacterial by topical or systematic approach. However, the effectiveness of antibacterial is diminishing because of the rapid emergence of antibiotic-resistant bacterial strains. Here, we designed and fabricated a silver nanoparticle (AgNPs)-based multicomponent hybrid nanocomposite termed as TSIIA @ CS/Lys @ AgNPs, which was comprised of a AgNPs core, a chitosan (CS) or lysozyme (Lys) middle layer, and a Tanshinone IIA (TSIIA) inclusion outlayer. Coating of CS or Lys to AgNPs through electrostatic interaction probably produced a core-shell nanocomplex resembling the endocarp of walnut. This design could reduce the dosage of AgNPs while maintaining antibacterial activity possibly due to the favorable interactions between nanocomplex and bacteria. The deposition of Chinese herb active component TSIIA by inclusion complexation formed the out layer of hybrid nanocomposite towards an improved antibacterial performance, which showed a therapeutic effect against acute otitis media of guinea pig comparable to the clinical commercial-used ofloxacin administrated by injection. The hybrid nanocomposite, when dispersed in poly (lactic-co-glycolic acid)/N-methyl-2-pyrrolidone (PLGA/NMP) solution as an in-situ organogel, not only maintained the therapeutic effectiveness, but also possessed the advantage of lower injection frequency compared with solution formulation. In addition, no obvious toxicity to the basilar membrane and epithelia tissue was observed after the healthy guinea pigs were treated with hybrid nanocomposite or organogel. This study provides a promising strategy to develop hybrid nanocomposite with enhanced antibacterial efficacy and also opens a new way for the establishment of efficient therapeutic systems with reduced administration frequency as substitute of antibiotics to treat otitis media.