The Anti-hyperlipidemia Effects of Raw Polygonum multiflorum Extract in Vivo
The Anti-hyperlipidemia Effects of Raw Polygonum multiflorum Extract in Vivo
复制标题
何首乌生提取物的体内抗高脂血症作用
DOI：
10.1248/bpb.b17-00218
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发表时间：
2017-11-01
影响因子：
2
通讯作者：
Xiao, Hongbin
中科院分区：
文献类型：
--
作者：
Xian, Zhong;Liu, Yan;Xiao, Hongbin
何首乌在中医中广泛用于高脂血症的防治。本研究探讨了生何首乌（RPM）调节血脂的作用及相关机制。结果表明，与模型组相比，RPM治疗组的基础血脂，如低密度脂蛋白胆固醇（LDL - C）、总胆固醇（TC）和甘油三酯（TG）显著降低，尤其是RPM高剂量组。口服给药后，血浆中参与脂质代谢的关键酶，3 - 羟基 - 3 - 甲基戊二酰辅酶A还原酶（HMGR）、脂肪酸合成酶（FAS）和乙酰辅酶A羧化酶（ACC）普遍降低，这与其靶基因的转录水平一致。此外，还研究了RPM的肝毒性，发现RPM所致的肝损伤比辛伐他汀诱导的略轻。组织学分析表明，高剂量（16.2 g/kg）口服RPM提取物后，肝细胞中的脂肪空泡和脂肪变性得到缓解，且比辛伐他汀诱导的效果更明显。这些结果表明，RPM通过调节相关基因的表达发挥其降脂作用，且在治疗高脂血症方面比辛伐他汀效果更好。
Polygonum multiflorum is widely used in the prevention and treatment of hyperlipidemia in traditional Chinese Medicine. In this study, the effects and relevant mechanisms of lipid-regulation by raw Polygonum multijlorum (RPM) were investigated. The results indicated that the basal plasma lipids, such as low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglycerides (TG), were significantly decreased in RPM treatment groups compared with the model group, especially in the RPM high dose group. The key enzymes involved in lipid metabolism, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) in plasma were generally reduced after oral administration, which was consistent with the transcription levels of their target genes. In addition, the hepatotoxicity of RPM was investigated, and RPM showed slightly less liver injury than that induced by simvastatin. Histological analysis indicated that the fat vacuoles and steatosis in hepatocytes were relieved after oral administration of RPM extract at a high dose of 16.2 g/kg, which was more obvious than that induced by simvastatin. These results revealed that RPM exerted its lipid-lowering effect by regulating the expression of related genes, and performed better than simvastatin in the treatment of hyperlipidemia.