Alveolar Bone Protective and Hypoglycemic Effects of Systemic Propolis Treatment in Experimental Periodontitis and Diabetes Mellitus
Alveolar Bone Protective and Hypoglycemic Effects of Systemic Propolis Treatment in Experimental Periodontitis and Diabetes Mellitus
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DOI：
10.1089/jmf.2013.3137
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发表时间：
2015-02-01
影响因子：
2.4
通讯作者：
Yakan, Birkan
中科院分区：
文献类型：
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作者：
Aral, Cuneyt Asim;Kesim, Servet;Yakan, Birkan
本研究的目的是评估的抗炎作用的效果，对实验性牙周炎和糖尿病的全身和局部的影响。将五十六只Wistar大鼠分为七组：（1）阴性对照组（NC），（2）牙周炎组（P），（3）糖尿病组（D），（4）糖尿病+牙周炎组（DP），（5）牙周炎+牙周炎组（P-Pro），（6）糖尿病+牙周炎组（D-Pro），（7）糖尿病+牙周炎+牙周炎组（DP-Pro）。牙周炎由结扎放置和糖尿病由链脲佐菌素注射诱导。蜂胶（Pro）通过经口管饲法（100 mg/kg/天）施用。在第21天，获得血浆用于分析，并使用组织形态计量学分析评估牙槽骨水平。与NC相比，D-Pro的最终血糖水平无显著差异（P= 0.052），但D、DP和DP-Pro存在显著差异。P和P-Pro之间、D和D-Pro之间以及DP和DP-Pro之间的血糖浓度无统计学显著差异。与NC相比，所有组均显示出显著更多的牙槽骨丢失。P与P-Pro、DP与DP-Pro之间的骨丢失差异有统计学意义，而D与D-Pro之间无差异。血浆白细胞介素1 β（IL-1 β）、肿瘤坏死因子-α（TNF-α）和基质金属蛋白酶-8（MMP-8）水平在各组间无显著差异。总之，降血糖可降低糖尿病患者的空腹血糖水平。此外，作为糖尿病相关性牙周炎和非糖尿病性牙周炎的辅助治疗，牙周炎可能是有益的。
The aim of this study was to evaluate the efficacy of the anti-inflammatory effects of propolis on the systemic and local effects on experimental periodontitis and diabetes. Fifty-six Wistar rats were divided into seven groups: (1) negative-control (NC), (2) periodontitis (P), (3) diabetes (D), (4) diabetes+periodontitis (DP), (5) periodontitis+propolis (P-Pro), (6) diabetes+propolis (D-Pro), and (7) diabetes+periodontitis+propolis (DP-Pro). Periodontitis was induced by ligature placement and diabetes was induced by streptozotocin injection. Propolis (Pro) was administrated by oral gavage (100 mg/kg/day). On day 21, plasma was obtained for analysis and alveolar bone level was evaluated using histomorphometric analysis. Compared to NC the final blood glucose levels for D-Pro was not significantly different (P=.052), however, D, DP, and DP-Pro were significantly different. There were no statistically significant differences in blood glucose concentrations between P and P-Pro, between D and D-Pro, and between DP and DP-Pro. All groups showed significantly more alveolar bone loss compared with NC. A significant difference in bone loss was found between P and P-Pro, and DP and DP-Pro, however there was no difference between D and D-Pro. Plasma interleukin 1beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and matrix metalloproteinase-8 (MMP-8) levels were not significantly different among groups. In conclusion, propolis reduced fasting blood glucose levels in diabetes. In addition, propolis might be beneficial as an adjunct treatment of diabetes associated periodontitis and periodontitis without diabetes.