Differences on Mediation of Calcium Oxalate Crystal Growth by Vero Cells before and after Repaired
Differences on Mediation of Calcium Oxalate Crystal Growth by Vero Cells before and after Repaired
复制标题
Vero细胞修复前后介导草酸钙晶体生长的差异
DOI：
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发表时间：
2012
影响因子：
0.7
通讯作者：
Ouyang Jian-Ming
中科院分区：
文献类型：
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作者：
Yao Xiu-Qiong;Peng Hua;Tan Jin;Ouyang Jian-Ming
The degradated soybean polysaccharides(SPS) were used to repair the injured African green monkey renal epithelial(Vero) cells,which were oxidatively injured by hydrogen peroxide(H2O2),by means of scanning electron microscopy(SEM) and flow cytometry etc.The differences on mediation of calcium oxalate(CaOxa) crystal growth by Vero cells before and after repaired were investigated.All the cellular viability,the activity of extracellular SOD and the intracellular mitochondrial membrane potential were increased after the repair by SPS to Vero cells.The morphology of injured Vero cells could be returned to plump state gradually after repaired.The repaired cells could reduce the formation of calcium oxalate monohydrate(COM) crystals with sharp edges and angles and increase the percentage of calcium oxalate dihydrate(COD) crystals.The size of the inductive CaOxa crystals by the three typological cells were as follows: normal cells repaired cells injured cells.The results in this study indicated that SPS could repair the injured Vero cells,thus reducing the risk of stone formation,and SPS may be a potential green anti-stone drug.
