CD62L expression level determines the cell fate of myeloid progenitors.
CD62L expression level determines the cell fate of myeloid progenitors.
复制标题
DOI：
10.1016/j.stemcr.2021.10.012
复制
发表时间：
2021-12-14
影响因子：
5.9
通讯作者：
Kurokawa M
中科院分区：
文献类型：
--
作者：
Ito Y;Nakahara F;Kagoya Y;Kurokawa M
造血细胞以分层方式分化为多个祖细胞，最近的单细胞分析揭示了每个祖细胞内的显着异质性。尽管共同骨髓祖细胞 (CMP) 被定义为可以分化为粒细胞-单核细胞祖细胞 (GMP) 和巨核细胞-红细胞祖细胞 (MEP) 的多能细胞群，并且 GMP 生成中性粒细胞和单核细胞，但这些骨髓祖细胞必须包含一些谱系定向祖细胞。通过单细胞水平的基因表达分析，我们确定 CD62L 作为揭示异质性的标记。我们证实，CD62L 阴性 CMP 代表“真正的”CMP，而 CD62L 高 CMP 大多仅限于小鼠和人类的 GMP 潜力。此外，我们还发现 CD62L 阴性 GMP 是 GMP 中最不成熟的亚群，Ly6C+CD62L-中型和 Ly6C+CD62L-高 GMP 分别偏向于小鼠的中性粒细胞和单核细胞分化。我们的发现有助于更深入地了解骨髓分化的机制。 CD62L-high CMP 在小鼠和人类中大多局限于 GMP 潜力 CD62L-neg GMP 在小鼠 GMP 中最不成熟 Ly6C+/CD62L-int GMP 偏向于小鼠中的中性粒细胞分化 Ly6C+/CD62L-high GMP 偏向于小鼠中的单核细胞分化 Kurokawa 及其同事将 CD62L 确定为揭示常见细胞异质性的标记物通过单细胞水平的基因表达分析来分析骨髓祖细胞 (CMP) 和粒细胞-单核细胞祖细胞 (GMP)。 CD62L-high CMP 主要局限于 GMP 潜力，而 Ly6C+/CD62L-int 和 -high GMP 分别偏向中性粒细胞和单核细胞分化。这些发现提供了对骨髓分化更深刻的理解。
Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent “bona fide” CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C+CD62L-intermediate and Ly6C+CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation. CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans CD62L-neg GMPs are most immature in murine GMPs Ly6C+/CD62L-int GMPs are skewed to neutrophil differentiation in mice Ly6C+/CD62L-high GMPs are skewed to monocyte differentiation in mice Kurokawa and colleagues identify CD62L as a marker to reveal the heterogeneity of common myeloid progenitors (CMPs) and granulocyte-monocyte progenitors (GMPs) through gene expression analysis at single-cell levels. CD62L-high CMPs are mostly restricted to GMP potentials, and Ly6C+/CD62L-int and -high GMPs are skewed to neutrophil and monocyte differentiation, respectively. These findings provide more profound understanding about myeloid differentiation.
登录
查看更多内容
DOI：
10.1073/pnas.1512076112
发表时间：
2015-08-04
影响因子：
11.1
作者：
Mori, Yasuo;Chen, James Y.;Weissman, Irving L.
通讯作者：
Weissman, Irving L.
DOI：
10.1002/stem.1985
发表时间：
2015-07
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Nishikii H;Kanazawa Y;Umemoto T;Goltsev Y;Matsuzaki Y;Matsushita K;Yamato M;Nolan GP;Negrin R;Chiba S
通讯作者：
Chiba S
影响因子：
24.8
作者：
Drissen, Roy;Thongjuea, Supat;Nerlov, Claus
通讯作者：
Nerlov, Claus
影响因子：
8
作者：
Cantor, AB;Orkin, SH
通讯作者：
Orkin, SH
影响因子：
11.4
作者：
Drexler, HG;Zaborski, M;Quentmeier, H
通讯作者：
Quentmeier, H