Kalirin and CHD7: novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria.
Kalirin and CHD7: novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria.
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DOI：
10.18632/oncotarget.14948
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发表时间：
2017-02-28
期刊：
影响因子：
--
通讯作者：
Barderas MG
中科院分区：
文献类型：
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作者：
de la Cuesta F;Baldan-Martin M;Moreno-Luna R;Alvarez-Llamas G;Gonzalez-Calero L;Mourino-Alvarez L;Sastre-Oliva T;López JA;Vázquez J;Ruiz-Hurtado G;Segura J;Vivanco F;Ruilope LM;Barderas MG
尽管抗高血压治疗取得了很大进展，但许多处于肾素-血管紧张素系统（RAS）抑制下的患者发生蛋白尿，这是治疗无效的明确指标。因此，需要血管功能的指标来评估患者的状况并帮助决定未来的治疗。循环细胞外囊泡（EV）的蛋白质组学分析显示，两种蛋白质，卡林和染色体结构域-解旋酶-DNA结合蛋白7（CHD 7），增加蛋白尿患者。在EV中发现两者与内皮活化标志物E-选择素的表达呈正相关。使用TNFα处理的成人内皮细胞进行的体外分析证明其参与内皮细胞活化。因此，我们建议循环EV中的kalirin和CHD 7蛋白水平作为新的内皮功能障碍标志物来监测高血压伴蛋白尿患者的血管状况。
Despite of the great advances in anti-hypertensive therapies, many patients under Renin-Angiotensin- System (RAS) suppression develop albuminuria, which is a clear indicator of therapeutic inefficiency. Hence, indicators of vascular function are needed to assess patients’ condition and help deciding future therapies. Proteomic analysis of circulating extracellular vesicles (EVs) showed two proteins, kalirin and chromodomain-helicase-DNA-binding protein 7 (CHD7), increased in albuminuric patients. A positive correlation of both with the expression of the endothelial activation marker E-selectin was found in EVs. In vitro analysis using TNFα-treated adult human endothelial cells proved their involvement in endothelial cell activation. Hence, we propose protein levels of kalirin and CHD7 in circulating EVs as novel endothelial dysfunction markers to monitor vascular condition in hypertensive patients with albuminuria.