NACP, a synaptic protein involved in Alzheimer's disease, is differentially regulated during megakaryocyte differentiation
NACP, a synaptic protein involved in Alzheimer's disease, is differentially regulated during megakaryocyte differentiation
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DOI：
10.1006/bbrc.1997.6978
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发表时间：
1997-08-28
影响因子：
3.1
通讯作者：
Masliah, E
中科院分区：
文献类型：
--
作者：
Hashimoto, M;Yoshimoto, M;Masliah, E
非淀粉样蛋白- β成分前体（NACP）是一种突触前蛋白，可能在阿尔茨海默病（AD）的淀粉样蛋白形成中起作用。由于血小板在AD中的异常功能已经被证实，血小板可以作为模型来研究NACP在该疾病中的作用。我们鉴定了巨核细胞-血小板系统（K562）中NACP和β -突触核蛋白的表达模式。在该造血细胞系中，在phorbol酯诱导的巨核细胞分化过程中，NACP表达上调，而β -突触核蛋白表达下调。与此一致的是，NACP而非β -突触核蛋白在血小板中大量表达。血小板免疫金电镜显示NACP与质膜、膜系统松散相关，偶尔也与分泌α -颗粒的膜相关。这些发现表明突触核蛋白家族成员的协调表达可能在造血细胞分化过程中发挥关键作用。此外，突触核蛋白家族成员的表达可能在神经分化过程中受到发育调节。(C) 1997学术出版社。
Non-amyloid-beta component precursor (NACP) is a presynaptic protein which may play a role in amyloidogenesis in Alzheimer's disease (AD). Since an abnormal function of platelets has been demonstrated in AD, platelets could be used as a model to investigate the role of NACP in this disease. We characterized the patterns of NACP and beta-synuclein expression in a megakaryocyte-platelet system (K562). In this hematopoietic cell line, NACP expression was up-regulated during phorbol ester-induced megakaryocytic differentiation, while beta-synuclein was down-regulated. Consistent with this, NACP but not beta-synuclein was abundantly expressed in platelets. Immunogold electron microscopy of platelets showed that NACP is loosely associated with the plasma membrane, the endomembrane system and, occasionally, with the membrane of secretory alpha-granules. These findings suggest that coordinate expression of the synuclein family members may play a critical role during hematopoietic cell differentiation. Additionally, expression of the synuclein family members may be developmentally regulated during neural differentiation. (C) 1997 Academic Press.