Rice allergenic protein and molecular-genetic approach for hypoallergenic rice.
Rice allergenic protein and molecular-genetic approach for hypoallergenic rice.
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大米过敏蛋白和低过敏性大米的分子遗传学方法。
DOI：
10.1271/bbb.60.1215
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Matsuda
中科院分区：
文献类型：
--
作者：
R. Nakamura;T. Matsuda
基于与来自对大米过敏的患者的IgE抗体的反应性，从大米盐可溶性级分中分离出分子量为约14至16 kDa的致敏蛋白。从成熟水稻种子的cDNA文库中分离出编码这些变应原蛋白的cDNA克隆，推导的氨基酸序列与小麦和大麦的α-淀粉酶/胰蛋白酶抑制剂具有相当大的相似性，后者最近被鉴定为与面包师哮喘相关的主要变应原。利用反义RNA技术抑制水稻成熟种子中过敏原基因的表达。免疫印迹和ELISA分析的种子使用单克隆抗体的16 kDa过敏原表明，过敏原含量的种子从几个转基因水稻植物显着低于亲本野生型水稻的种子。
Allergenic proteins with a molecular mass of about 14 to 16 kDa were isolated from a rice salt-soluble fraction based on the reactivity with IgE antibodies from patients allergic to rice. cDNA clones encoding these allergenic proteins were isolated from a cDNA library of maturing rice seeds, and the deduced amino acid sequences showed considerable similarity to wheat and barley alpha-amylase/trypsin inhibitors, which have recently been identified as major allergens associated with baker's asthma. An antisense RNA strategy was applied to repress the allergen gene expression in maturing rice seeds. Immunoblotting and ELISA analyses of the seeds using a monoclonal antibody to a 16-kDa allergen showed that allergen content of seeds from several transgenic rice plants was markedly lower than that of the seeds from parental wild type rice.