Characterization and nucleotide sequence of the gene for the common alpha subunit of the bovine pituitary glycoprotein hormones.
Characterization and nucleotide sequence of the gene for the common alpha subunit of the bovine pituitary glycoprotein hormones.
复制标题
牛垂体糖蛋白激素常见α亚基基因的特征和核苷酸序列。
DOI：
10.1093/nar/11.19.6873
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发表时间：
1983
影响因子：
14.9
通讯作者：
Nilson,JH
中科院分区：
文献类型：
--
作者：
Goodwin,RG;Moncman,CL;Rottman,FM;Nilson,JH
从牛基因组文库中分离到牛垂体糖蛋白激素共同α亚基的编码基因。该基因跨度约16.5 kbp，包含三个中间序列，编码约730个核苷酸的信息。对该基因的完整编码区以及5'侧序列和整个内含子c的315个核苷酸进行了测序，与cDNA序列比较发现只有一个碱基的差异。基因组印迹实验表明存在单个α亚基基因。牛α亚基基因与人α亚基基因的比较表明，在5′和3′非翻译区以及5′侧区至少90个核苷酸的编码区保持着高度的同源性。此外，在该基因的5‘侧区和5’非翻译区都存在一个18个碱基对序列，该序列与鸡卵白蛋白基因的一个区域同源。这个卵清蛋白序列被认为是黄体酮受体复合物的结合位点。
The gene coding for the common α subunit of the bovine pituitary glycoprotein hormones was isolated from a bovine genomic library. The gene spans roughly 16.5 kbp, contains three intervening sequences, and codes for a message of approximately 730 nucleotides. The complete coding region of the gene was sequenced as well as 315 nucleotides of 5' flanking sequence and the entire intron C. Only a single base difference was found when the sequence of the gene was compared with that of the cDNA. Genomic blotting experiments suggest the presence of a single α subunit gene. Comparison of the bovine and human α subunit genes indicated that the high level of homology observed in the coding regions has been maintained throughout the 5' and 3' untranslated regions, and at least 90 nucleotides of the 5'flanking regions. Additionally, there is an 18 base pair sequence present in both the 5' flanking and 5' untranslated regions of the gene that is homologous to a region of the chick ovalbumin gene. This ovalbumin sequence has been suggested as a binding site for the progesterone receptor-complex.