Horizontal transfer and functional evaluation of high pathogenicity islands in Avian Escherichia coli
Horizontal transfer and functional evaluation of high pathogenicity islands in Avian Escherichia coli
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禽大肠杆菌高致病性岛的水平转移及功能评价
DOI：
10.1515/pjvs-2017-0048
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发表时间：
2017-01-01
影响因子：
0.8
通讯作者：
Zhu, L.
中科院分区：
文献类型：
--
作者：
Tu, J.;Qi, K.;Zhu, L.
大肠杆菌中的高致病性岛（HPI）编码主要参与铁吸收和调节的基因，并赋予毒力和致病性。本研究的目的是研究HPI在禽大肠杆菌中的转移。coli中表达，并鉴定HPI在受体菌中的功能。在低温、低铁丰度条件下获得了HPI转移菌株，并对供体和受体菌株进行了确认。E.大肠杆菌HPI通过水平基因转移事件转移，这可能主要由HPI相邻序列中的同源重组介导。对受体菌株的生物学活性和致病性变化的分析表明，HPI可能不参与禽大肠杆菌的致病。因此，HPI在该细菌菌株中的主要功能可能是调节铁营养。
High pathogenicity islands (HPIs) in Escherichia coli encode genes that are primarily involved in iron uptake and regulation, and confer virulence and pathogenicity. The aim of this study was to investigate the transfer of HPIs in avian E. coli and identify the function of HPI in the acceptor strain. The HPI transfer strain was obtained under conditions of low temperature and low iron abundance, and the donor and acceptor strains were confirmed. E. coli HPIs are transferred by horizontal gene transfer events, which are likely mediated primarily by homologous recombination in HPI-adjacent sequences. Assays for biological activity and pathogenicity changes in the acceptor strain indicated that HPIs might not be involved in pathogenesis in avian E. coli, and thus the main function of HPIs in this strain of bacteria may be to regulate iron nutrition.