Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp lycopersici, and its races
Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp lycopersici, and its races
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DOI：
10.1007/s10327-010-0224-7
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发表时间：
2010-04-01
影响因子：
1.2
通讯作者：
Arie, Tsutomu
中科院分区：
文献类型：
--
作者：
Inami, Keigo;Yoshioka, Chizu;Arie, Tsutomu
5对用于番茄枯萎病病原鉴定的引物/探针。番茄及其三个小种是根据rDNA基因间隔区和无毒基因选择性设计的。利用菌丝体基因组DNA和土壤DNA的实时荧光定量聚合酶链式反应(Real-Time PCR，RT-PCR)成功地鉴定了FOL及其小种。
Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.