Symbiont Identity Influences Patterns of Symbiosis Establishment, Host Growth, and Asexual Reproduction in a Model Cnidarian-Dinoflagellate Symbiosis
Symbiont Identity Influences Patterns of Symbiosis Establishment, Host Growth, and Asexual Reproduction in a Model Cnidarian-Dinoflagellate Symbiosis
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DOI：
10.1086/696365
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发表时间：
2018-02-01
影响因子：
1.6
通讯作者：
Davy, Simon K.
中科院分区：
文献类型：
--
作者：
Gabay, Yasmin;Weis, Virginia M.;Davy, Simon K.
共生藻属是生理多样性的，因此可能差异影响共生的建立和功能。为了探索这一点，我们接种了海葵Exaiptasia pallida（通常称为“Aiptasia”）的非共生个体，这是一种珊瑚共生的模型，与五种共生藻种或类型之一（S。microadriaticum、小亚德里亚链霉菌S. minutum、C3型、S. trenchii或S. voratum）。通过共聚焦显微镜随时间监测定殖的空间模式，并测量各种生理参数以评估共生功能。银莲花与同源共生体S. minutum，但与共生藻C3或S. voratum，分别。微adriaticum和S. trenchii的成功，但在S.分钟殖民化的空间格局是相同的所有共生藻类群，最终取得成功，开始在口腔盘和发展的触角，入侵之前的列，最后，踏板盘。在所有情况下，通过海葵的触手增殖是斑片状的，这表明共生体被驱逐到胃血管腔，并重新被宿主吞噬。然而，这些不同的空间事件的时间不同的共生藻类群之间。此外，S. microadriaticum和S. trenchii对宿主的益处较少，表现为光合作用、海葵生长和足裂伤的速率较低。这项研究增强了我们对共生体身份与整体共生关系之间联系的理解，这对于理解新型宿主-共生体配对的潜在建立和持久性非常重要。重要的是，我们还提供了一个基线，为进一步研究这一主题与全球采用的“Aiptasia”模型系统。
The genus Symbiodinium is physiologically diverse and so may differentially influence symbiosis establishment and function. To explore this, we inoculated aposymbiotic individuals of the sea anemone Exaiptasia pallida (commonly referred to as "Aiptasia"), a model for coral symbiosis, with one of five Symbiodinium species or types (S. microadriaticum, S. minutum, phylotype C3, S. trenchii, or S. voratum). The spatial pattern of colonization was monitored over time via confocal microscopy, and various physiological parameters were measured to assess symbiosis functionality. Anemones rapidly formed a symbiosis with the homologous symbiont, S. minutum, but struggled or failed to form a long-lasting symbiosis with Symbiodinium C3 or S. voratum, respectively. Symbiodinium microadriaticum and S. trenchii were successful but reached their peak density two weeks after S. minutum. The spatial pattern of colonization was identical for all Symbiodinium taxa that were ultimately successful, starting in the oral disk and progressing to the tentacles, before invading the column and, finally, the pedal disk. In all cases, proliferation through the anemone's tentacles was patchy, suggesting that symbionts were being expelled into the gastrovascular cavity and re-phagocytosed by the host. However, the timing of these various spatial events differed between the different Symbiodinium taxa. Furthermore, S. microadriaticum and S. trenchii were less beneficial to the host, as indicated by lower rates of photosynthesis, anemone growth, and pedal laceration. This study enhances our understanding of the link between symbiont identity and the performance of the overall symbiosis, which is important for understanding the potential establishment and persistence of novel host-symbiont pairings. Importantly, we also provide a baseline for further studies on this topic with the globally adopted "Aiptasia" model system.