A Simple and Low-Cost Procedure for Growing Geobacter sulfurreducens Cell Cultures and Biofilms in Bioelectrochemical Systems.
A Simple and Low-Cost Procedure for Growing Geobacter sulfurreducens Cell Cultures and Biofilms in Bioelectrochemical Systems.
复制标题
DOI：
10.1002/cpmc.20
复制
发表时间：
2016-11-18
影响因子：
--
通讯作者：
Malvankar NS
中科院分区：
文献类型：
--
作者：
O'Brien JP;Malvankar NS
厌氧微生物在多种环境过程中发挥着核心作用，并调节营养物和矿物质的全球生物地球化学循环。许多厌氧微生物对于生物能源和生物燃料的生产非常重要。然而，在实验室研究厌氧微生物的主要障碍是需要复杂且昂贵的加油站和手套箱来创建和维持厌氧环境。本附录介绍了一种简单的加气站设计，经验不足的研究人员可以轻松使用它来培养厌氧微生物。此外，本附录还详细介绍了生物电化学系统的低成本组装，并概述了使用硫还原地杆菌作为模型生物体培养和分析产生电流的细菌细胞培养物和生物膜的简化程序。
Anaerobic microorganisms play a central role in several environmental processes and regulate global biogeochemical cycling of nutrients and minerals. Many anaerobic microorganisms are important for the production of bioenergy and biofuels. However, the major hurdle in studying anaerobic microorganisms in the laboratory is the requirement for sophisticated and expensive gassing stations and glove boxes to create and maintain the anaerobic environment. This appendix presents a simple design for a gassing station that can be used readily by an inexperienced investigator for cultivation of anaerobic microorganisms. In addition, this appendix also details the low-cost assembly of bioelectrochemical systems and outlines a simplified procedure for cultivating and analyzing bacterial cell cultures and biofilms that produce electric current, using Geobacter sulfurreducens as a model organism.