Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip.
Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip.
复制标题
DOI：
10.1007/s12195-012-0240-0
复制
发表时间：
2012-09-01
影响因子：
2.8
通讯作者：
Gao, Bruce Z.
中科院分区：
文献类型：
--
作者：
Ma, Zhen;Liu, Qiuying;Liu, Honghai;Yang, Huaxiao;Yun, Julie X.;Xu, Meifeng;Eisenberg, Carol A.;Borg, Thomas K.;Markwald, Roger;Gao, Bruce Z.
正常心肌细胞高度依赖于离子通道的功能表达来形成动作电位和与其他细胞的电耦合。为了充分确定干细胞用于心血管疾病治疗的科学和治疗潜力，有必要全面评估干细胞-心肌细胞相互作用期间干细胞电特性的调节。已有文献报道，与天然心肌细胞接触可诱导和调节干细胞的心源性分化。然而，在常规的细胞培养模型中，由于对单个细胞的细胞接触模式的控制不足，细胞-细胞接触对于干细胞与心肌细胞的功能偶联的重要性尚未阐明。使用微加工和激光引导细胞微图案化技术，我们创建了两个生物芯片与接触促进和预防微环境系统地研究接触对干细胞电特性的心源性调节的影响。在接触促进生物芯片中，连接蛋白43的表达上调，并重新定位到一个干细胞和一个心肌细胞之间的连接区域。只有与心肌细胞接触的干细胞才能被相邻的心肌细胞诱导获得类似于心肌细胞的动作电位形成的电生理特性。
Normal cardiomyocytes are highly dependent on the functional expression of ion channels to form action potentials and electrical coupling with other cells. To fully determine the scientific and therapeutic potential of stem cells for cardiovascular-disease treatment, it is necessary to assess comprehensively the regulation of stem-cell electrical properties during stem cell-cardiomyocyte interaction. It has been reported in the literature that contact with native cardiomyocytes induced and regulated stem-cell cardiogenic differentiation. However, in conventional cell-culture models, the importance of cell-cell contact for stem-cell functional coupling with cardiomyocytes has not been elucidated due to insufficient control of the cell-contact mode of individual cells. Using microfabrication and laser-guided cell micropatterning techniques, we created two biochips with contact-promotive and -preventive microenvironments to systematically study the effect of contact on cardiogenic regulation of stem-cell electrical properties. In contact-promotive biochips, connexin 43 expression was upregulated and relocated to the junction area between one stem cell and one cardiomyocyte. Only stem cells in contact with cardiomyocytes were induced by adjacent cardiomyocytes to acquire electrophysiological properties for action-potential formation similar to that of a cardiomyocyte.
登录
查看更多内容
影响因子：
--
作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
生物芯片/激光细胞沉积系统，用于评估具有新颖不对称几何形状的微通道中单个神经元和神经元/胶质细胞对的偏振轴突生长
影响因子：
3.2
作者：
Pirlo, R. K.;Sweeney, A. J.;Gao, B. Z.
通讯作者：
Gao, B. Z.
影响因子：
6.1
作者：
Revzin, A;Sekine, K;Toner, M
通讯作者：
Toner, M
影响因子：
6.1
作者：
Di Carlo, Dino;Wu, Liz Y.;Lee, Luke P.
通讯作者：
Lee, Luke P.
DOI：
10.1152/ajpheart.91531.2007
发表时间：
2008-07-01
影响因子：
4.8
作者：
Pedrotty, Dawn M.;Klinger, Rebecca Y.;Bursac, Nenad
通讯作者：
Bursac, Nenad