RNA structural constraints in the evolution of the influenza A virus genome NP segment
RNA structural constraints in the evolution of the influenza A virus genome NP segment
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DOI：
10.4161/rna.29730
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发表时间：
2014-07-01
期刊：
影响因子：
4.1
通讯作者：
Olsthoorn, Rene C. L.
中科院分区：
文献类型：
--
作者：
Gultyaev, Alexander P.;Tsyganov-Bodounov, Anton;Olsthoorn, Rene C. L.
利用比较序列和结构分析预测了甲型流感病毒基因组核蛋白（NP）片段的保守RNA二级结构。在整个片段长度上，包括蛋白质编码区，鉴定了许多显示核苷酸协变的结构元件。对配对核苷酸位置的互信息值的计算表明，这些结构对病毒基因组的进化施加了相当大的限制。假结结构在NP包装信号区域的功能重要性被预测，并通过对结构破坏的突变病毒和使用代偿取代恢复折叠的突变病毒的斑块分析得到证实。讨论了甲型流感病毒基因组中保守RNA折叠模式的可能功能。
Conserved RNA secondary structures were predicted in the nucleoprotein (NP) segment of the influenza A virus genome using comparative sequence and structure analysis. A number of structural elements exhibiting nucleotide covariations were identified over the whole segment length, including protein-coding regions. Calculations of mutual information values at the paired nucleotide positions demonstrate that these structures impose considerable constraints on the virus genome evolution. Functional importance of a pseudoknot structure, predicted in the NP packaging signal region, was confirmed by plaque assays of the mutant viruses with disrupted structure and those with restored folding using compensatory substitutions. Possible functions of the conserved RNA folding patterns in the influenza A virus genome are discussed.