Poly(lactide-co-glycolide)/titania Composite Microsphere-Sintered Scaffolds for Bone Tissue Engineering Applications
Poly(lactide-co-glycolide)/titania Composite Microsphere-Sintered Scaffolds for Bone Tissue Engineering Applications
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DOI：
10.1002/jbm.b.31561
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Wang, Dong-An
中科院分区：
文献类型：
--
作者：
Wang, Yingjun;Shi, Xuetao;Wang, Dong-An
本研究的目的是合成和表征新型的三维多孔支架制成的聚（乳酸-羟基乙酸）（PLGA）/纳米二氧化钛颗粒复合微球的潜在骨修复应用。已证明引入TiO 2组分能够大大增强PLGA/TiO 2微球烧结支架（“PLGA/TiO 2-SMS”）的机械性能。此外，复合纳米TiO 2添加剂能够诱导来自环境的粘附蛋白的增加的停滞，这有利于细胞附着到支架上。用MTT法、碱性磷酸酶（ALP）活性测定、骨钙化测定等方法检测成骨细胞的增殖和成熟情况。结果表明，与纯PLGA支架相比，在具有不同TiO 2含量（0、0.1和0.3g/lg PLEA）的复合支架上培养的成骨细胞显示出增加的细胞增殖。当在复合支架上培养时，成骨细胞也表现出显著增强的ALP活性和更高的钙分泌，相对于纯PLGA支架上的成骨细胞。总之，PLGA/TiO 2-SMSs值得关注，可用于潜在的骨修复治疗。(C)2010 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appf Biomater 93 B：84-92。2010
The objective of this study was to synthesize and characterize novel three-dimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO2-particle composite microspheres for potential bone repair applications. The introduction of TiO2 component has been proven capable of largely enhancing mechanical properties of PLGA/TIO2 microsphere-sintered scaffold ("PLGA/TiO2-SMS"). In addition, composite nano-TiO2 additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO2 content (0, 0.1, and 0.3 g/l g PLEA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO2-SMSs deserve attention utilizing for potential bone-repairing therapeutics. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appf Biomater 93B: 84-92. 2010