TOPOGRAPHY OF CHOLINERGIC AFFERENTS FROM THE NUCLEUS BASALIS OF MEYNERT TO REPRESENTATIONAL AREAS OF SENSORIMOTOR CORTICES IN THE RAT
TOPOGRAPHY OF CHOLINERGIC AFFERENTS FROM THE NUCLEUS BASALIS OF MEYNERT TO REPRESENTATIONAL AREAS OF SENSORIMOTOR CORTICES IN THE RAT
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DOI：
10.1002/cne.903350407
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发表时间：
1993-09-22
影响因子：
2.5
通讯作者：
HERRON, P
中科院分区：
文献类型：
--
作者：
BASKERVILLE, KA;CHANG, HT;HERRON, P
本实验观察了大鼠Meynert基底核（NBM）内向初级躯体感觉（SI）、次级躯体感觉（SII）和初级运动（MI）皮质限制区传出投射神经元的分布，以及这些投射神经元中胆碱能神经元的百分比。（3）单个NBM神经元与SI内不同亚部、SI和SII的同伦区以及SI和MI的同伦区的侧支化（如果有的话）。逆行单和双标记技术被用来研究NBM预测电生理学确定的细分SI和同伦代表领域的SI和SII，SI和MI。胆碱乙酰转移酶免疫细胞化学鉴定胆碱能NBM神经元。在投射到SI、SII和MI的选择性细分的逆行标记的NBM神经元中，分别有89%、87%和88%是胆碱能的。我们发现了一个rostral-to-caudal的进展后，逆行标记的NBM神经元的内侧到外侧的序列注射到细分的SI。重叠组的单标记NBM神经元注射后观察到不同的示踪剂到相邻的细分内SI或同伦领域的SI和SII，SI和MI。我们的结论是，NBM神经支配SI，SII，和MI主要是胆碱能在大鼠，每个皮质区接收胆碱能传入神经元广泛分布在NBM内，每个NBM神经元项目到一个有限的皮质区没有显着的抵押相邻的细分内SI或同伦领域的SI和SII，或SI和MI。 (C)1993 Wiley-Liss，Inc.
We investigated (1) the topography of projection neurons in the nucleus basalis of Meynert (NBM) with efferents to restricted regions of the primary somatosensory (SI), the second somatosensory (SII), and the primary motor (MI) cortices in the rat; (2) the percentage of these NBM projection neurons that were cholinergic; and (3) the collateralization, if any, of single NBM neurons to different subdivisions within SI, to homotopic areas of SI and SII, and to homotopic areas of SI and MI. Retrograde single- and double-labeling techniques were used to study NBM projections to electrophysiologically identified subdivisions of SI and to homotopic representational areas of SI and SII, and of SI and MI. Choline acetyltransferase immunocytochemistry was done to identify cholinergic NBM neurons. Of the retrogradely labeled NBM neurons that projected to selective subdivisions of SI, SII, and MI, 89%, 87%, and 88%, respectively, were cholinergic. We found a rostral-to-caudal progression of retrogradely labeled NBM neurons following a medial-to-lateral sequence of injections into subdivisions of SI. Overlapping groups of single-labeled NBM neurons were observed after injections of different tracers into adjacent subdivisions within SI or homotopic areas of SI and SII, and of SI and MI. We conclude that NBM innervation to SI, SII, and MI is mostly cholinergic in the rat, that each cortical area receives cholinergic afferents from neurons widely distributed within the NBM, and that each NBM neuron projects to a restricted cortical area without significant collateralization to adjacent subdivisions within SI or to homotopic areas of SI and SII, or SI and MI. (C) 1993 Wiley-Liss, Inc.