Antimicrobial susceptibility and genetic characterisation of Burkholderia pseudomallei isolated from Malaysian patients.
Antimicrobial susceptibility and genetic characterisation of Burkholderia pseudomallei isolated from Malaysian patients.
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DOI：
10.1155/2014/132971
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Vadivelu, Jamuna
中科院分区：
文献类型：
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作者：
Khosravi, Yalda;Vellasamy, Kumutha Malar;Vadivelu, Jamuna
假性伯克霍尔德菌是类鼻疽病的病原体，对许多抗生素具有内在耐药性。头孢他啶（CAZ）是一种合成的β -内酰胺，通常被用作治疗类鼻疽的一线抗生素。然而，在CAZ治疗期间，获得性CAZ耐药性可在体内发展，如果不及时切换到不同的抗生素治疗，将导致死亡。本研究采用纸片扩散法、肉汤微量稀释法和Etest法测定81株假假芽孢杆菌对9种不同抗菌药物的敏感性。对CAZ、阿莫西林/克拉维酸、美罗培南、亚胺培南和甲氧苄啶/磺胺甲恶唑的敏感性最高。尽管这些药物在假芽孢杆菌中显示出最高的敏感性百分比，但总体结果强调了这种生物中出现耐药性的重要性。PCR结果显示，81株假假杆菌中有6株多药耐药菌株携带bpeB、amrB、BPSS1119和penA基因。利用随机扩增多态性DNA分析对分离株进行基因分型，结果显示从6株MDR分离株聚类(A)中产生6种不同的PCR指纹图谱，从其余75株非MDR分离株聚类(B)中产生8种不同的PCR指纹图谱。
Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics. Ceftazidime (CAZ), the synthetic beta-lactam, is normally used as the first-line antibiotic therapy for treatment of melioidosis. However, acquired CAZ resistance can develop in vivo during treatment with CAZ, leading to mortality if therapy is not switched to a different antibiotic(s) in a timely manner. In this study, susceptibilities of 81 B. pseudomallei isolates to nine different antimicrobial agents were determined using the disk diffusion method, broth microdilution test and Etest. Highest percentage of susceptibility was demonstrated to CAZ, amoxicillin/clavulanic acid, meropenem, imipenem, and trimethoprim/sulfamethoxazole. Although these drugs demonstrated the highest percentage of susceptibility in B. pseudomallei, the overall results underline the importance of the emergence of resistance in this organism. PCR results showed that, of the 81 B. pseudomallei, six multidrug resistant (MDR) isolates carried bpeB, amrB, and BPSS1119 and penA genes. Genotyping of the isolates using random amplified polymorphic DNA analysis showed six different PCR fingerprinting patterns generated from the six MDR isolates clusters (A) and eight PCR fingerprinting patterns generated for the remaining 75 non-MDR isolates clusters (B).