Application of Methane Mixed Plasma for the Determination of Ge, As, and Se in Serum and Urine by ICP/MS
Application of Methane Mixed Plasma for the Determination of Ge, As, and Se in Serum and Urine by ICP/MS
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DOI：
10.5012/bkcs.2003.24.3.285
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发表时间：
2003
影响因子：
1.7
通讯作者：
K. Park;Sun-Tae Kim;Youngman Kim;Yunje Kim;W. Lee
中科院分区：
文献类型：
--
作者：
K. Park;Sun-Tae Kim;Youngman Kim;Yunje Kim;W. Lee
建立了电感耦合等离子体质谱法同时测定生物样品中痕量锗、砷和硒的分析方法。为了提高检测限、准确度和精密度，研究了在冷却剂氩气中加入有机气体对分析物信号的影响。在冷却剂气体通道中加入少量甲烷（10 mL/min.）可以改善Ge、As和Se的电离。所提出的Ar/ ch4体系的分析灵敏度比传统的Ar方法至少高出两倍。该方法对Ge、As和Se的检出限分别为0.014、0.012和0.064 μg/L。通过对标准物质（SRM）的分析，评价了该方法的分析可靠性。用NIST SRM法对冻干尿样的Ge、As和Se的回收率分别为99.9%、103%和96.5%。该方法也适用于生物样品。
An analytical method for the simultaneous determination of trace Ge, As and Se in biological samples by inductively coupled plasma/mass spectrometry has been investigated. The effects of added organic gas into the coolant argon gas on the analyte signal were studied to improve the detection limit, accuracy and precision. The addition of a small amount of methane (10 mL/min.) into the coolant gas channel improved the ionization of Ge, As and Se. The analytical sensitivity of the proposed Ar/CH 4 system was superior by at least two-fold to that of the conventional Ar method. In the present method, the detection limits obtained for Ge, As and Se were 0.014, 0.012 and 0.064 μg/L, respectively. The analytical reliability of the proposed method was evaluated by analyzing the certified standard reference materials (SRM). Recoveries of 99.9% for Ge, 103% for As, 96.5% for Se were obtained for NIST SRM of freeze dried urine sample. The proposed method was also applied to the biological samples.