Double stranded RNA- relative to other TLR ligand-activated dendritic cells induce extremely polarized human Th1 responses
Double stranded RNA- relative to other TLR ligand-activated dendritic cells induce extremely polarized human Th1 responses
复制标题
DOI：
10.1016/j.cellimm.2010.05.008
复制
发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Lee, David R.
中科院分区：
文献类型：
--
作者：
Benwell, Risa K.;Hruska, Jennifer E.;Lee, David R.
为了更好地了解使用不同TLR配体激活的树突状细胞诱导人CD4(+) T淋巴细胞反应的相对效率，我们用两种病毒和两种细菌TLR配体激活了人树突状细胞，并产生了il - 12。虽然两种病毒TLR配体（ssRNA和dsRNA）诱导DC产生可检测水平的IL12p70，但两种细菌TLR配体（LPS和鞭毛蛋白）激活的DC诱导人类同种异体原生CD4(+) T细胞增殖增加，dsRNA激活的DC诱导Th1增加和Th2分化减少。与未刺激和其他TLR配体激活的dc相比，IL12p70的中和消除了所有TLR配体激活的moDCs诱导的大部分Th1偏斜。总之，这些结果表明，dsrna激活的dc诱导的人类Th1反应比其他TLR配体激活的dc更高度极化。这些结果对TLR配体在免疫治疗中的应用具有重要意义。(C) 2010 Elsevier Inc .版权所有
To better understand the relative efficiencies of using different TLR ligand-activated DCs to induce human CD4(+) T lymphocyte responses, human DCs were activated with two viral and two bacterial TLR ligands, and Melt production of IL12. TNF alpha, and IL10 was examined While the two viral TLR ligands (ssRNA and dsRNA) induced DC production of detectable levels of IL12p70, DCs activated by the two bacterial TLR ligands (LPS and flagellin) induced increased proliferation of human allogeneic naive CD4(+) T cells dsRNA-activated DCs induced increased Th1 and decreased Th2 differentiation, resulting in extremely polarized responses relative to those induced by unstimulated and other TLR ligand-activated DCs Neutralization of IL12p70 abrogated most of the Th1 skewing induced by all TLR ligand-activated moDCs Collectively, these results demonstrate that dsRNA-activated DCs induce more highly polarized human Th1 responses than the other TLR ligand-activated DCs tested here These results have implications for TLR ligands in immunotherapy. (C) 2010 Elsevier Inc All rights reserved