TRANSACTIVATION AND DNA-BINDING PROPERTIES OF THE TRANSCRIPTION FACTOR, SOX-18
TRANSACTIVATION AND DNA-BINDING PROPERTIES OF THE TRANSCRIPTION FACTOR, SOX-18
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DOI：
10.1093/nar/23.14.2626
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发表时间：
1995-07-25
影响因子：
14.9
通讯作者：
DUNN, TL
中科院分区：
文献类型：
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作者：
HOSKING, BM;MUSCAT, GEO;DUNN, TL
SOX-18是SOX多基因家族的成员(Sry相关的HMG-box基因)，我们已经在细菌中表达了这个378个氨基酸的蛋白，并证明了它与Sox DNA结合基序AACAAAG的序列特异性结合。利用GAL4-SOX-18融合蛋白(160-225个氨基酸)，在SOX-18中定位了一个独特的95个氨基酸的激活区。此外，SOX-18能够通过AACAAAG基序反式激活基因表达。我们的结果表明，SOX-18是一种经典的基因表达反式激活因子。
Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene), We have bacterially expressed this 378 amino acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160-225). Furthermore, Sox-18 was capable of trans-activating gene expression through the AACAAAG motif. Our results suggest that Sox-18 functions as a classical trans-activator of gene expression.