Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A/PMA
Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A/PMA
复制标题
DOI：
10.1006/fsim.2002.0448
复制
发表时间：
2003-05-01
影响因子：
4.7
通讯作者：
Aoki, T
中科院分区：
文献类型：
--
作者：
Nam, BH;Hirono, I;Aoki, T
在这项研究中，表达序列标签测序与消减杂交相结合，在用刀豆球蛋白 (Con) A/佛波醇肉豆蔻酸酯 (PMA) 作为体外激活系统处理后，从日本牙鲆 (P. olivaceus) 外周血白细胞中筛选生物防御或免疫反应相关基因。数据库搜索确定了 43 个假定的免疫反应相关基因，这些基因在 Con A/PMA 刺激后表达。这些假定的免疫反应相关基因分为6组：凋亡相关基因（3个基因）、细胞因子（7个基因）、细胞因子受体（3个基因）、信号转导因子（5个基因）、转录因子（11个基因）和其他（14个基因）。结果表明，鱼类白细胞受到哺乳动物有丝分裂原的模拟，并在受到哺乳动物有丝分裂原的刺激后发生增殖。在增殖过程中，它们表达并利用各种细胞因子基因或免疫反应相关基因进行细胞之间的通讯或信号转导或细胞内信号级联。
In the study, expressed sequence tag sequencing has been coupled with subtractive hybridization to screen for biodefence or immune response-related genes from Japanese flounder (P. olivaceus) peripheral blood leukocytes after treatment with Concanavalin (Con) A/phorbol myristate acetate (PMA) as an in vitro activation system. Database searches identified 43 putative immune response-related genes that were expressed following Con A/PMA stimulation. These putative immune response-related genes are classified into 6 groups: apoptosis-related genes (3 genes), cytokines (7 genes), cytokine receptors (3 genes), signal transduction factors (5 genes), transcription factors (11 genes), and others (14 genes). Results show that fish leukocytes are simulated by, and undergo proliferation following stimulation by mammalian mitogens. During proliferation, they express and utilize various cytokine genes or immune response related genes for communication or signal transduction between cells or intracellular signalling cascades.