METHODS FOR DIETARY FIBER, NEUTRAL DETERGENT FIBER, AND NONSTARCH POLYSACCHARIDES IN RELATION TO ANIMAL NUTRITION
METHODS FOR DIETARY FIBER, NEUTRAL DETERGENT FIBER, AND NONSTARCH POLYSACCHARIDES IN RELATION TO ANIMAL NUTRITION
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DOI：
10.3168/jds.s0022-0302(91)78551-2
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发表时间：
1991-10-01
影响因子：
3.5
通讯作者：
LEWIS, BA
中科院分区：
文献类型：
--
作者：
VANSOEST, PJ;ROBERTSON, JB;LEWIS, BA
需要标准化NDF程序。 由于使用了不同的淀粉酶来试图去除淀粉干扰，因此程序也有所不同。 最初的枯草芽孢杆菌酶IIIA型（XIA）不再可用，已被一种效率较低的酶取代。 对于纤维工作，一种新的酶1已获得AOAC批准，并在分析工作中迅速取代其他淀粉酶。 该酶可从Sigma（编号A3306; Sigma Chemical Co.，圣路易斯，密苏里州）。 NDF和ADF的原始出版物（43，53）和农业手册379（14）已经过时，仅具有历史意义。 应遵循最新程序。 由于报告的毒性，三甘醇已取代2-乙氧基乙醇。 在过去的5年里，由于对人类和单胃动物膳食纤维的重新定义，包括木质素和所有对哺乳动物消化酶有抗性的多糖，纤维方法有了相当大的发展。 除了NDF之外，现在还有新的改进方法用于总膳食纤维和非淀粉多糖，包括果胶和β-葡聚糖。 后者在瘤胃发酵中也有意义。 与淀粉不同，它们的发酵类似于纤维素的发酵，但更快，不产生乳酸。 碳水化合物组分的物理和生物性质比其内在组成更重要。
There is a need to standardize the NDF procedure. Procedures have varied because of the use of different amylases in attempts to remove starch interference. The original Bacillus subtilis enzyme Type IIIA (XIA) no longer is available and has been replaced by a less effective enzyme. For fiber work, a new enzyme1 has received AOAC approval and is rapidly displacing other amylases in analytical work. This enzyme is available from Sigma (Number A3306; Sigma Chemical Co., St. Louis, MO). The original publications for NDF and ADF (43, 53) and the Agricultural Handbook 379 (14) are obsolete and of historical interest only. Up to date procedures should be followed. Triethylene glycol has replaced 2-ethoxyethanol because of reported toxicity. Considerable development in regard to fiber methods has occurred over the past 5 yr because of a redefinition of dietary fiber for man and monogastric animals that includes lignin and all polysaccharides resistant to mammalian digestive enzymes. In addition to NDF, new improved methods for total dietary fiber and nonstarch polysaccharides including pectin and beta-glucans now are available. The latter are also of interest in rumen fermentation. Unlike starch, their fermentations are like that of cellulose but faster and yield no lactic acid. Physical and biological properties of carbohydrate fractions are more important than their intrinsic composition.