Effect of gastrin on differentiation of rat intestinal epithelial cells in vitro.
Effect of gastrin on differentiation of rat intestinal epithelial cells in vitro.
复制标题
胃泌素对大鼠肠上皮细胞体外分化的影响。
DOI：
10.3748/wjg.v9.i8.1786
复制
发表时间：
2003
影响因子：
4.3
通讯作者：
Jingchun Sun
中科院分区：
文献类型：
--
作者：
Zhou Wang;Wei;Ruliu Li;B. Wen;Jingchun Sun
目的
探讨胃泌素对IEC-6细胞体外分化的影响。
方法
IEC-6细胞与胃泌素一起孵育。处理后第7天，光学显微镜观察细胞形态，第20天，电子显微镜观察细胞超微结构。在胃泌素作用6 h后，用逆转录-聚合酶链反应（RT-PCR）检测绒毛蛋白mRNA的表达，第7天用激光共聚焦显微镜观察绒毛蛋白的表达。
结果
经胃泌素处理后，IEC-6细胞表现为绒毛状肠上皮细胞，胞浆丰富，细胞核小，核仁多，排列规则。细胞边缘有大量微绒毛，部分细胞呈柱状结构。细胞质中绒毛蛋白mRNA的表达较对照组增加。
结论
胃泌素可诱导肠绒毛细胞的分化特征和大鼠肠上皮细胞（IEC-6）的表型变化，且胃泌素的作用与绒毛蛋白表达增加相关。
AIM
To investigate the effect of gastrin on differentiation of IEC-6 cell line in vitro.
METHODS
IEC-6 cells were incubated with gastrin. On day 7 after treatment, cell morphology was examined by light microscope, and on day 20, the cellular ultrastructures were examined by electron microscope. After exposure to gastrin for 6 hours, villin mRNA was analyzed by reverse transcription-polymerase chain reaction, and on day 7, the expression of villin was examined by immunocytochemical analysis with laser confocal microscope.
RESULTS
After exposure to gastrin, IEC-6 cells showed differentiated phenotypes as villas enterocytes and contained an abundance of plasma, small nuclei with nucleoli, and were arranged regularly. There were numerous microvilli around edge of the cells, and several cells showed columnar structures. Villin mRNA expression in cytoplasm was increased in comparison with control.
CONCLUSION
Differentiated characteristics of villus enterocytes and phenotypic changes of rat intestinal epithelial cells (IEC-6) are induced by gastrin, and the effects of gastrin are correlated to increased villin expression.
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DOI：
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期刊：
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影响因子：
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American journal of physiology. Cell physiology
影响因子：
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