Association between outcome and telomere DNA content in prostate cancer
Association between outcome and telomere DNA content in prostate cancer
复制标题
DOI：
10.1016/s0022-5347(05)68239-0
复制
发表时间：
1999-11-01
影响因子：
6.6
通讯作者：
Griffith, J
中科院分区：
文献类型：
--
作者：
Donaldson, L;Fordyce, C;Griffith, J
目的：为初步探讨器官局限性前列腺癌患者的预后与肿瘤细胞端粒DNA含量的关系，材料与方法：病例对照研究组为1993年以前诊断为前列腺癌的18例男性患者。这组被选择为大约一半在诊断后10年内死亡，另一半存活10年或更长时间。对每例患者回收存档的石蜡包埋肿瘤组织。从新切的切片中提取DNA，固定在尼龙膜上，与P-32标记的着丝粒和端粒特异性探针杂交。用放射性同位素杂交法定量分析端粒DNA含量，探讨端粒DNA含量与生存率、端粒DNA含量与肿瘤复发的关系。和疾病复发与端粒DNA含量减少有关（p
Purpose: To perform an initial retrospective investigation of the relationship between outcome in patients with organ confined prostate adenocarcinoma and the tumor cells' content of telomere DNA,Materials and Methods: The case-controlled study group was composed of eighteen men diagnosed with prostatic adenocarcinoma prior to 1993. The group was selected so that approximately one half died within ten years of diagnosis and one half survived ten years or longer. Archival, paraffin-embedded tumor tissue was recovered for each patient. DNA was extracted from newly cut sections, fixed to nylon membranes and hybridized with P-32-labeled centromere-and telomere-specific probes. Telomere DNA contents were quantitated from the hybridized radioactivities, The relationships between telomere DNA content and survival, and telomere DNA content and disease recurrence in men receiving prostatectomies were determined.Results: Death. and disease recurrence were associated with reduced telomere DNA content (p