Highly pathogenic H5N1 influenza virus causes coagulopathy in chickens
Highly pathogenic H5N1 influenza virus causes coagulopathy in chickens
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DOI：
10.1111/j.1348-0421.2006.tb03764.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Muramoto, Y;Ozaki, H;Kida, H
在感染高致病性禽流感（HPAI） A型病毒的鸡中，经常观察到多器官严重出血。在这项研究中，我们研究了HPAI病毒感染是否会导致鸡凝血障碍。病理检查显示，经静脉感染高致病性禽流感病毒的鸡在肺小动脉内形成纤维蛋白血栓，与内皮细胞的病毒抗原有关。鸡外周血的血液学分析显示，当病毒抗原仅在内皮细胞和单核/巨噬细胞中检测到时，凝血功能在感染早期就开始了。此外，HPAI病毒感染鸡的脾脏、肺和脑中组织因子的基因表达上调，组织因子是血液凝固的主要引发剂。这些结果提示，感染HPAI病毒后，内皮细胞和单核/巨噬细胞功能障碍可能导致鸡出现以过度凝血和消耗性凝血功能障碍为代表的止血异常。
Severe hemorrhage at multiple organs is frequently observed in chickens infected with highly pathogenic avian influenza (HPAI) A viruses. In this study we examined whether HPAI virus infection leads to coagulation disorder in chickens. Pathological examinations showed that the fibrin thrombi were formed in arterioles at the lung, associated with the viral antigens in endothelial cells of chickens infected intravenously with HPAI virus. Hematological analyses of peripheral blood collected from the chickens revealed that coagulopathy was initiated at early stage of infection when viral antigens were detected only in the endothelial cells and monocytes/macrophages. Furthermore, gene expression of the tissue factor, the main initiator of blood coagulation, was upregulated in the spleen, lung, and brain of HPAI virus-infected chickens. These results suggest that dysfunction of endothelial cells and monocytes/macrophages upon HPAI virus infection may induce hemostasis abnormalities represented by the excessive blood coagulation and consumptive coagulopathy in chickens.