Modified lactic acid bacteria detect and inhibit multiresistant enterococci.
Modified lactic acid bacteria detect and inhibit multiresistant enterococci.
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DOI：
10.1021/sb500090b
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发表时间：
2015-03-20
影响因子：
4.7
通讯作者：
Kaznessis, Yiannis N.
中科院分区：
文献类型：
--
作者：
Borrero, Juan;Chen, Yuqing;Dunny, Gary M.;Kaznessis, Yiannis N.
关键词：
我们设计了乳酸乳球菌来检测粪肠球菌。经检测，L.乳酸菌产生并分泌抗肠球菌肽。这些肽抑制肠球菌的生长并降低L.乳酸菌。肠球菌性信息素cCF10作为检测信号。以cCF 10应答性大肠杆菌pCF 10为载体构建表达载体。faecalis性信息素接合质粒，在L.用于检测系统的乳酸。重组宿主菌株被工程化以表达三种细菌素的基因，肠菌素A、hiracin JM 79和肠菌素P，每一种都具有对E.粪便。在琼脂和液体培养基中均可进行灵敏检测和特异性抑制。工程L.乳酸菌还能抑制多药耐药大肠杆菌的生长。屎肠菌菌株，当诱导cCF10。所提出的载体和菌株可以是工具箱的组成部分，用于开发针对感染部位肠球菌的替代抗生素技术。
We designed Lactococcus lactis to detect Enterococcus faecalis. Upon detection, L. lactis produce and secrete antienterococcal peptides. The peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of L. lactis. The enterococcal sex pheromone cCF10 serves as the signal for detection. Expression vectors derived from pCF10, a cCF10-responsive E. faecalis sex-pheromone conjugative plasmid, were engineered in L. lactis for the detection system. Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis. Sensitive detection and specific inhibition occur both in agar and liquid media. The engineered L. lactis also inhibited growth of multidrug-resistant E. faecium strains, when induced by cCF10. The presented vectors and strains can be components of a toolbox for the development of alternative antibiotic technologies targeting enterococci at the site of infection.
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影响因子：
4.4
作者：
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通讯作者：
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DOI：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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