SIMPLE, ONE-STEP CHROMATOGRAPHIC PROCEDURE FOR PURIFICATION OF LYSOZYME
SIMPLE, ONE-STEP CHROMATOGRAPHIC PROCEDURE FOR PURIFICATION OF LYSOZYME
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DOI：
10.1016/0005-2744(78)90045-1
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
RODRIGUEZ, R
中科院分区：
文献类型：
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作者：
FERNANDEZSOUSA, JM;PEREZCASTELLS, R;RODRIGUEZ, R
A rapid method for the purification of lysozyme (mucopeptide N-acetylmuramoylhydrolase, EC 3.2.1.17) from hen egg-white was devised. It was that gel filtration chromatography on agarose columns can be used selectively to purify lysozyme, dut to the fact that this protein interacts with the agarose matrix and elutes later than the corresponding total volume for the column. Thus, lysozyme is directly obtained in a relatively pure form and with a high specific activity. In principle, this simple method can be used to prepare lysozymes from other sources.
