DdaSTE12 is involved in trap formation, ring inflation, conidiation, and vegetative growth in the nematode-trapping fungus Drechslerella dactyloides
DdaSTE12 is involved in trap formation, ring inflation, conidiation, and vegetative growth in the nematode-trapping fungus Drechslerella dactyloides
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DdaSTE12 参与捕获线虫的真菌 Drechslerella dactyloides 中的陷阱形成、环膨胀、分生孢子和营养生长
DOI：
10.1007/s00253-021-11455-z
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发表时间：
2021-09-18
影响因子：
5
通讯作者：
Liu, Xingzhong
中科院分区：
文献类型：
--
作者：
Fan, Yani;Zhang, Weiwei;Liu, Xingzhong
Ste 12转录因子是丝裂原活化蛋白激酶（MAPK）信号通路的下游，仅在真菌界中发现，并调节真菌的交配，发育和致病性。捕食线虫的真菌Drechsleella dacquitides可以利用收缩环捕获自由生活的线虫，当线虫进入环时，在1 s内细胞膨胀。丝裂原活化蛋白激酶信号通路参与捕食线虫真菌的陷阱形成，但其下游调控尚不清楚。在本研究中，DdaSTE 12基因在D. daclidides使收缩环的细胞膨胀失效并导致不能捕获线虫。DeltaDdaSTE 12的收缩环隔膜和环细胞空泡的数量发生了变化。与野生型相比，DeltaDdaSTE 12的诱捕器形成、分生孢子形成和营养生长分别减少了79.3%、80.3%和21.5%。与野生型相比，Delta DdaSTE 12 -3的转录组表明，参与陷阱形成过程的基因的表达，包括信号转导（Gpa 2和7-跨膜受体），囊泡运输和细胞融合（含MARVEL结构域的蛋白质），和线虫感染（PEX 11和CFEM结构域的蛋白质），由DdaSTE 12调节。结果表明，DdaSTE 12参与陷阱的形成和环形细胞膨胀，以及分生孢子和营养生长，通过调节广泛的下游功能。我们的研究结果扩大了Ste 12同源转录因子在收缩环的发展中的作用，并为线虫捕食中MAPK信号通路的下游调控提供了新的见解。
Ste12 transcription factors, downstream of mitogen-activated protein kinase (MAPK) signalling pathways, are exclusively found in the fungal kingdom and regulate fungal mating, development, and pathogenicity. The nematode-trapping fungus Drechslerella dactyloides can capture free-living nematodes using constricting rings by cell inflation within 1 s when stimulated by nematodes entering the rings. The MAPK signalling pathways are involved in the trap formation of nematode-trapping fungi, but their downstream regulation is not clearly understood. In this study, disruption of the DdaSTE12 gene in D. dactyloides disabled cell inflation of constricting rings and led to an inability to capture nematodes. The number of septa of constricting rings and the ring cell vacuoles were changed in Delta DdaSTE12. Compared with the wild type, Delta DdaSTE12 reduced trap formation, conidiation, and vegetative growth by 79.3%, 80.3%, and 21.5%, respectively. The transcriptomes of Delta DdaSTE12-3, compared with those of the wild type, indicated that the expression of genes participating in trap formation processes, including signal transduction (Gpa2 and a 7-transmembrane receptor), vesicular transport and cell fusion (MARVEL domain-containing proteins), and nematode infection (PEX11 and CFEM domain-containing proteins), is regulated by DdaSTE12. The results suggest that DdaSTE12 is involved in trap formation and ring cell inflation, as well as conidiation and vegetative growth, by regulating a wide range of downstream functions. Our findings expanded the roles of Ste12 homologous transcription factors in the development of constricting rings and provided new insights into the downstream regulation of the MAPK signalling pathway involved in nematode predation.