Black TiO2 nanotubes: Efficient electrodes for triggering electric field-induced stimulation of stem cell growth.
Black TiO2 nanotubes: Efficient electrodes for triggering electric field-induced stimulation of stem cell growth.
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DOI：
10.1016/j.actbio.2019.08.021
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发表时间：
2019-10
影响因子：
9.7
通讯作者：
A. Mazare;Jung Park;S. Simons;S. Mohajernia;I. Hwang;J. E. Yoo;H. Schneider;Michael J M Fischer;Patrik Schmuki
中科院分区：
文献类型：
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作者：
A. Mazare;Jung Park;S. Simons;S. Mohajernia;I. Hwang;J. E. Yoo;H. Schneider;Michael J M Fischer;Patrik Schmuki
二氧化钛纳米结构兼具生物相容性和高比表面积，是生物医学应用的重要平台。尤其是二氧化钛纳米管层由于具有可控的纳米拓扑学效应，在电刺激实验中用作电极也得到了广泛的研究。在目前的工作中，我们制备了Ar/H2还原的具有强烈增强电导率的黑色TiO2纳米管阵列，并探索了它们作为电极时与间充质干细胞的相互作用，以在细胞中施加电场(EF)。虽然我们没有观察到细胞在这些高导电性纳米管上的粘附性和焦点接触形成的显著变化，但我们确实观察到当EF使用黑色的TiO2纳米管阵列作为电极时，干细胞迅速做出反应。与形成的纳米管阵列相比，观察到干细胞生长更快，较低的EF强度导致细胞内钙水平升高。我们的结果表明，二氧化钛纳米管的导电性增加显著增强了早期干细胞对最小电场刺激的反应。意义陈述二氧化钛纳米结构在生物医学应用中的应用被广泛研究，特别是考虑到纳米结构表面对生物材料-细胞相互作用的影响。我们之前已经证明，在没有生化骨诱导补充剂的情况下，对生长在二氧化钛纳米管上的干细胞施加电场(EF)可以产生协同成骨刺激。在这里，我们报告了黑色(即通过还原处理获得的高导电性纳米管)TiO2纳米管对干细胞的短期EF效应：我们观察到干细胞生长更快，对最小EF刺激的早期干细胞反应显著增强。这种纳米结构在电场下的应用有望用于骨再生的治疗干预和组织工程方法。
TiO2nanostructures represent a key platform for biomedical applications, due to the combination of biocompatibility and high surface area. Especially TiO2nanotube layers have been widely investigated due to controllable nanotopographic effects as well as for electrodes in electrostimulation experiments. In the present work we produce Ar/H2-reduced ‘black’ TiO2nanotube arrays with a strongly enhanced electrical conductivity and explore their interaction with mesenchymal stem cells when used as electrodes to apply electric fields (EF) across the cells. While we observe no significant change in cell adhesion and their focal contact formation on these high conductivity nanotubes, we do observe a rapid stem cell response when EF is engaged using the ‘black’ TiO2nanotube arrays as electrodes. Compared to as-formed nanotube arrays, a faster stem cell growth was observed and a lower EF intensity caused an intracellular calcium level elevation. Our results indicate that the increased conductivity in TiO2nanotubes significantly enhances the early stem cell response to minimal electric field stimuli.Statement of SignificanceThe use of TiO2nanostructures in biomedical applications is widely investigated, especially considering the nanostructured surface influence on the biomaterial-cell interactions. We have previously shown that an applied electric field (EF) on stem cells grown on TiO2nanotubes leads to synergistic osteogenic stimulation in the absence of biochemical bone-inducing supplements. Here we report that black (i.e. highly conductive nanotubes obtained by reduction treatments) TiO2nanotubes enable short-time EF effects on stem cells: we observe a faster stem cell growth and a significantly enhanced early stem cell response to minimal EF stimuli. The application of such nanostructures under electric field is promising for therapeutic interventions for bone regeneration and tissue engineering approaches.