White light excitation fluorescence (WLEF). Part II. Analysis of complex multifluorophoric systems.
White light excitation fluorescence (WLEF). Part II. Analysis of complex multifluorophoric systems.
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白光激发荧光 (WLEF) 第二部分。
DOI：
10.1039/c0ay00704h
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发表时间：
2011
影响因子：
3.1
通讯作者：
A. Mishra
中科院分区：
文献类型：
--
作者：
J. Prakash;A. Mishra
探讨了白色光激发荧光分析多荧光体系的适用范围。白色光激发荧光（WLEF）光谱保留了所有荧光团及其相互作用的所得荧光特征，尽管与激发-发射-基质荧光（EEMF）光谱相反，沿激发轴的分辨率沿着丢失。WLEF在光纤兼容性、灵敏度、选择性和同时检测多个荧光团方面的优势的证明表明，基于WLEF的分析技术在某些情况下可以是选择的方法，特别是在分析物的低成本原位分析方面。使用光纤荧光计通过WLEF对多荧光体系的分析清楚地显示了WLEF的有效性：（i）在低荧光团浓度下的双荧光体系中，其中光谱加和性被保留;（ii）在复杂的多荧光体系中，如柴油-煤油混合物。
The scope of white light excitation fluorescence in analyzing multifluorophoric systems has been explored. White light excitation fluorescence (WLEF) spectra retain the resultant fluorescence signature of all the fluorophores and their interactions although, in contrast to excitation-emission-matrix fluorescence (EEMF) spectra, resolution along the excitation axis is lost. The demonstration of the advantages of WLEF with regard to fiber optic compatibility, sensitivity, selectivity and simultaneous detection of multiple fluorophores suggests that WLEF based analytical techniques can be methods of choice in certain cases, especially with regard to low cost in situ analysis of an analyte. The analysis of multifluorophoric systems by WLEF using a fiber optic fluorimeter clearly shows the effectiveness of WLEF (i) in two-fluorophoric systems at low fluorophore concentrations in which the spectral additivity is retained and (ii) in a complex multifluorophoric system like diesel-kerosene mixtures.