A DNA nanomachine that maps spatial and temporal pH changes inside living cells
A DNA nanomachine that maps spatial and temporal pH changes inside living cells
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DOI：
10.1038/nnano.2009.83
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发表时间：
2009-05-01
影响因子：
38.3
通讯作者：
Krishnan, Yamuna
中科院分区：
文献类型：
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作者：
Modi, Souvik;Swetha, M. G.;Krishnan, Yamuna
DNA纳米机器是一种合成组装体，在外部触发器的刺激下，它可以在定义的分子构象之间切换。此前，DNA设备的性能仅限于体外应用。在这里，我们报告了一种称为I开关的DNA纳米机器的构建，它由质子触发，并基于活细胞内的荧光共振能量转移（FRET）作为PH传感器。它是pH 5.5至6.8的有效报告物，在pH 5.8至7之间具有高动态范围。为了证明其在活细胞内发挥作用的能力，我们使用I开关来绘制与内体成熟相关的空间和时间pH变化。我们的DNA纳米器件在生命系统中的性能说明了DNA支架在生命系统中对更复杂的传感，诊断和靶向治疗触发器做出反应的潜力。
DNA nanomachines are synthetic assemblies that switch between defined molecular conformations upon stimulation by external triggers. Previously, the performance of DNA devices has been limited to in vitro applications. Here we report the construction of a DNA nanomachine called the I-switch, which is triggered by protons and functions as a PH sensor based on fluorescence resonance energy transfer (FRET) inside living cells. It is an efficient reporter of pH from PH 5.5 to 6.8, with a high dynamic range between pH 5.8 and 7. To demonstrate its ability to function inside living cells we use the I-switch to map spatial and temporal pH changes associated with endosome maturation. The performance of our DNA nanodevices inside living systems illustrates the potential of DNA scaffolds responsive to more complex triggers in sensing, diagnostics and targeted therapies in living systems.