Antioxidative and antimutagenic activity of yeast cell wall mannans in vitro
Antioxidative and antimutagenic activity of yeast cell wall mannans in vitro
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DOI：
10.1016/s1383-5718(01)00257-1
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发表时间：
2001-10-18
影响因子：
1.9
通讯作者：
Krajcovic, J
中科院分区：
文献类型：
--
作者：
Krizková, L;Duracková, Z;Krajcovic, J
研究了酵母细胞壁甘露聚糖的抗氧化和抗诱变作用，特别是来自念珠菌、酿酒酵母和白色念珠菌的细胞外葡甘露聚糖（EC-GM）和葡甘露聚糖（gm - c.u.）的抗氧化和抗诱变作用。以trolox为标准品的鲁米诺依赖性光化学方法表明，EC-GM、GM-C.u。, M-S.c。和M-C.a。具有较好的抗氧化性能。EC-GM的抗氧化活性最高，GM-C.u次之。和M-S.c。M-C.a。抗氧化活性最低。实验证实，这些甘露聚糖在减轻氧氟沙星和吖啶橙（AO）对鞭毛虫叶绿草（Euglena gracilis）叶绿体DNA的损伤方面表现出不同的抗诱变活性，具有统计学意义。我们认为EC-GM、gm - c.u.的抗诱变作用较弱。, M-S.c。和M-C.a。氧氟沙星是基于它们清除活性氧自由基的能力。在AO处理下，叶绿体DNA损伤的减少可能是甘露聚糖吸收能力的结果。从酵母细胞壁中分离出的甘露聚糖具有良好的水溶性、相对较小的分子量（15-30 kDa）以及通过不同的作用方式发挥的抗诱变作用等重要特性，这似乎是它们作为天然保护（抗诱变）剂的一个有前景的特征。(C) 2001 Elsevier Science B.V.版权所有
Antioxidative and antimutagenic effect of yeast cell wall mannans, in particular, extracellular glucomannan (EC-GM) and glucomannan (GM-C.u.) both from Candida utilis, mannan from Saccharomyces cerevisiae (M-S.c.) and mannan from Candida albicans (M-C.a.) was evaluated. Luminol-dependent photochemical method using trolox as a standard showed that EC-GM, GM-C.u., M-S.c. and M-C.a. have relatively good antioxidative properties. EC-GM exhibited the highest antioxidative activity, followed by GM-C.u. and M-S.c. M-C.a. showed the least antioxidative activity. These mannans were experimentally confirmed to exhibit different, statistically significant antimutagenic activity in reducing damage of chloroplast DNA of the flagellate Euglena gracilis induced by ofloxacin and acridine orange (AO). We suggest that the antimutagenic effect of EC-GM, GM-C.u., M-S.c. and M-C.a. against ofloxacin is based on their ability to scavenge reactive oxygen radicals. With AO, the reduction of the chloroplast DNA lession could be a result of the absorptive capacity of the mannans. The important characteristics of mannans isolated from the yeast cell walls, such as good water solubility, relatively small molecular weight (15-30 kDa), and antimutagenic effect exerted through different mode of action, appear to be a promising features for their prospective use as a natural protective (antimutagenic) agents. (C) 2001 Elsevier Science B.V. All rights reserved.