EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA)/CYCLIN DURING THE CELL-CYCLE
EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA)/CYCLIN DURING THE CELL-CYCLE
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DOI：
10.1016/0014-4827(86)90520-3
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发表时间：
1986-09-01
影响因子：
3.7
通讯作者：
TAN, EM
中科院分区：
文献类型：
--
作者：
KURKI, P;VANDERLAAN, M;TAN, EM
增殖细胞核抗原（PCNA），也称为细胞周期蛋白的表达，定量的细胞系SP2/0和MOLT-4和小鼠脾细胞诱导增殖体外有丝分裂原。用来自系统性红斑狼疮患者的自身抗体标记细胞悬液中的细胞增殖细胞核抗原（PCNA）。在相同的细胞中，DNA用碘化丙啶染色。然后通过流式细胞术分析细胞的PCNA和DNA含量。增殖期脾细胞和细胞系的PCNA表达谱相似。G 0-G1期细胞PCNA表达不明显。在拉格G1期细胞中观察到PCNA免疫荧光显着增加，并且在S期细胞中观察到进一步增加。G2-M期细胞PCNA免疫荧光强度较S期细胞降低，但仍高于G 0-G1期细胞。与未受刺激的细胞相比，通过暴露于阿糖胞苷而在G1-S边界被捕的增殖细胞显示出增加的PCNA免疫荧光。
The expression of proliferating cell nuclear antigen (PCNA), also called cyclin, was quantified in the cell lines SP2/0 and MOLT-4 and in mouse splenocytes induced to proliferate in vitro with mitogens. Autoantibody from a patient with systemic lupus erythematosus was used to label PCNA in cell suspensions after the cells had been fixed and permeabilized. In the same cells DNA was stained by propidium iodide. The cells were then analysed by flow cytometry for PCNA and DNA content. The PCNA profiles in proliferating spleen cells and the cell lines were similar. Most G0-G1 cells did not express significant amount of PCNA. A dramatic increase in PCNA immunofluorescence was observed in lage G1 cells, and further increases were observed in S-phase cells. G2-M cells showed a reduced level of PCNA immunoflourescence relative to S-phase cells but were still elevated relative to G0-G1 cells. Proliferating cells arrested at the G1-S boundary by exposure to cytosine arabinoside showed an increased PCNA immunoflourescence as compared to unstimulated cells.