Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli
Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli
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DOI：
10.1016/s1046-5928(02)00609-5
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发表时间：
2003-02-01
影响因子：
1.6
通讯作者：
Wu, CF
中科院分区：
文献类型：
--
作者：
Liu, YF;Ma, RL;Wu, CF
根据东亚钳蝎（Buthus martensii Karsch）毒抗肿瘤镇痛肽（AGAP）的cDNA序列，通过PCR技术获得了该肽的编码基因，并在大肠杆菌中表达。而重组AGAP大部分以不溶性包涵体的形式表达。重组AGAP通过金属螯合亲和层析纯化至均一。药理试验表明，重组AGAP对小鼠具有镇痛和抗肿瘤活性。(C)2002 Elsevier Science（美国）。All rights reserved.
The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice. (C) 2002 Elsevier Science (USA). All rights reserved.