The ATPase FliI can interact with the type III flagellar protein export apparatus in the absence of its regulator, FliH
The ATPase FliI can interact with the type III flagellar protein export apparatus in the absence of its regulator, FliH
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DOI：
10.1128/jb.185.13.3983-3988.2003
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发表时间：
2003-07-01
影响因子：
3.2
通讯作者：
Macnab, RM
中科院分区：
文献类型：
--
作者：
Minamino, T;González-Pedrajo, B;Macnab, RM
FliI沙门氏菌是驱动鞭毛蛋白输出的atp酶。它通常与调节蛋白FliH以复合体的形式存在。一个fliH零突变体有轻微的运动性，由于填充的过量产生导致其运动性的显著改善。FlhA和FlhB是III型鞭毛输出装置的完整膜组分，其细胞质结构域的突变也导致了运动性的显著改善，即使在正常的FliI水平下也是如此。因此，FliH虽然无疑很重要，但并不是必不可少的。
Salmonella FliI is the ATPase that drives flagellar protein export. It normally exists as a complex together with the regulatory protein FliH. A fliH null mutant was slightly motile, with overproduction of Fill resulting in substantial improvement of its motility. Mutations in the cytoplasmic domains of FlhA and FlhB, which are integral membrane components of the type III flagellar export apparatus, also resulted in substantially improved motility, even at normal FliI levels. Thus, FliH, though undoubtedly important, is not essential.