Molecular analysis of commensal host-microbial relations hips in the intestine
Molecular analysis of commensal host-microbial relations hips in the intestine
复制标题
DOI：
10.1126/science.291.5505.881
复制
发表时间：
2001-02-02
期刊：
影响因子：
56.9
通讯作者：
Gordon, JI
中科院分区：
文献类型：
--
作者：
Hooper, LV;Wong, MH;Gordon, JI
人类包含复杂的本土微生物社会，但对常驻细菌如何塑造我们的生理机能知之甚少。我们用多形拟杆菌（Bacteroides thetaiotaomicron）定殖无菌小鼠，多形拟杆菌是正常小鼠和人类肠道微生物菌群的重要组成部分。用DNA微阵列观察了全球肠道对定植的转录反应，并通过激光捕获显微切割建立了选定反应的细胞起源。结果表明，这种肠道细菌调节参与几个重要的肠道功能，包括营养吸收，粘膜屏障强化，异生物质代谢，血管生成和出生后肠道成熟的基因的表达。这些发现提供了关于常驻微生物与其宿主之间相互作用的本质的观点。
Human beings contain complex societies of indigenous microbes, yet little is known about how resident bacteria shape our physiology. We colonized germfree mice with Bacteroides thetaiotaomicron, a prominent component of the normal mouse and human intestinal microflora. Global intestinal transcriptional responses to colonization were observed with DNA microarrays, and the cellular origins of selected responses were established by laser-capture microdissection. The results reveal that this commensal bacterium modulates expression of genes involved in several important intestinal functions, including nutrient absorption, mucosal barrier fortification, xenobiotic metabolism, angiogenesis, and postnatal intestinal maturation. These findings provide perspectives about the essential nature of the interactions between resident microorganisms and their hosts.