LC-MS determination and pharmacokinetic study of six phenolic components in rat plasma after taking traditional Chinese medicinal-preparation: Guanxinning lyophilized powder for injection
LC-MS determination and pharmacokinetic study of six phenolic components in rat plasma after taking traditional Chinese medicinal-preparation: Guanxinning lyophilized powder for injection
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DOI：
10.1016/j.jchromb.2008.07.039
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发表时间：
2008-09-15
影响因子：
3
通讯作者：
Bi, Kaishun
中科院分区：
文献类型：
--
作者：
Guo, Xiaorui;Chen, Xiaohui;Bi, Kaishun
以丹参为原料制成注射用中药制剂冠心宁冻干粉。(SMB)和川芎(Ligusticum川雄)。(LCH)进行了研究。为了了解冻干粉的动力学行为，为合理给药提供依据，我们建立了一种灵敏、重现性好的高效液相色谱-质谱法测定冠心宁中6种主要酚类成分[丹参素(DSS)、原儿茶酸(PAC)、原儿茶醛(PAL)、绿原酸(CHA)、咖啡酸(CAA)和丹酚酸B(SAB)]在大鼠血浆中的含量及药代动力学。样品制备采用蛋白质沉淀法，加入甲醇，加入内标(大蒜酸)后，用乙酸乙酯-乙醚(3：1，v/v)液液萃取。蒸发至干燥后，生成的残留物在甲醇中重组成，并进样到Kromasil C-18柱(150 mm x 4.6 mm i.d.)上。颗粒大小为5微米)。在选择离子监测(SIM)模式下，使用负电喷雾电离(ESI)质谱仪对分析物进行分析。DSS、PAC、PAL、CHA、CAA、SAB的线性范围分别为0.342~85.0、0.0647~12.9、0.0933~18.7、0.0085~3.4、0.0138~2.75、0.0272~810mgmL(-1)。6种分析物从大鼠血浆中的平均提取回收率均不低于75%，精密度和准确度均在规定范围内。将该方法成功地用于冠心宁冻干粉的6种酚类成分在大鼠体内的药代动力学研究。皇冠版权所有(C)2008由爱思唯尔出版。保留所有权利。
A traditional Chinese medicinal preparation (TCMP) named Guanxinning lyophilized powder for injection composed of Salvia miltiorrhiza Bge. (SMB) and Ligusticum chuanxiong Hort. (LCH) was studied. In order to learn the kinetic behaviors of the lyophilized powder and provide proofs for rational administration, we have developed a sensitive and reproducible method for determination and pharmacokinetic study of six main phenolic components {danshensu (DSS), protocatechuic acid (PAC), protocatechuic aldehyde (PAL), chlorogenic acid (CHA), caffeic acid (CAA) and salvianolic acid B (SAB)} of Guanxinning in rat plasma using liquid chromatography-mass spectrometric (LC-MS) method. Sample preparations were carried out by protein precipitation with the addition of methanol followed by liquid-liquid extraction with ethyl acetate-ethyl ether (3:1, v/v) after internal standard (IS, galic acid) spiked. After evaporation to dryness, the resultant residue was reconstituted in methanol and injected onto a Kromasil C-18 column (150 mm x 4.6 mm i.d. with 5 mu m particle size). The analytes were analyzed by using negative electrospray ionization (ESI) mass spectrometry in selected ion monitoring (SIM) mode. The method was with good linearity in the range 0.342-85.0 mu g mL(-1) for DSS, 0.0647-12.9 mu g mL(-1) for PAC, 0.0933-18.7 mu g mL(-1) for PAL, 0.0085-3.40 mu g mL(-1) for CHA, 0.0138-2.75 mu g mL(-1) for CAA and 0.0272-810 mu g mL(-1) for SAB (r > 0.99). The average extract recoveries of the six analytes from rat plasma were all no less than 75%, the precision and accuracy determined were all within the required limits. This LC-MS method was successfully applied to pharmacokinetic study of the six phenolic components of Guanxinning lyophilized powder for injection in rats. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.