Isolation of chicken vitellogenin I and III cDNAs and the developmental regulation of five estrogen-responsive genes in the embryonic liver.
Isolation of chicken vitellogenin I and III cDNAs and the developmental regulation of five estrogen-responsive genes in the embryonic liver.
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DOI：
10.1101/gad.2.1.116
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发表时间：
1988
影响因子：
10.5
通讯作者：
M. Evans;Rosane Silva;J. Burch
中科院分区：
文献类型：
--
作者：
M. Evans;Rosane Silva;J. Burch
报道了鸡卵黄蛋白原（VTG）两个次要基因（VTGI和VTGIII）部分编码的cDNA克隆的分离。这些克隆代表了独特的序列，这些序列只在雌性化鸟类的肝脏中表达。在产蛋母鸡的肝脏中，编码vttgi、VTGII和VTGIII的rna水平分别约为每个细胞11000、30000和3000分子。我们使用新分离的克隆，以及先前可用的卵黄蛋白cdna [VTGII，载脂蛋白II （apoVLDLII）和载脂蛋白B]作为探针，研究雌二醇对这些基因调控的几个方面。首先，我们证明了每个基因对雌二醇的反应能力是在卵子的8到13天之间获得的。其中四种基因对雌二醇的反应在胎儿发育后期减弱，但在孵化后一周内恢复。其次，我们证明这些基因在加入雌二醇后表现出不同的动力学反应谱。第三，正如之前对VTGII和apoVLDLII基因所描述的那样，我们证明单次注射雌二醇会影响长期重编程事件（肝脏记忆），这使得在随后的雌二醇再挑战后，VTGII和VTGIII rna的快速积累更快。总的来说，这三组数据表明，分子参数可能有助于雌二醇对这组基因的坐标和非坐标调节。
The isolation of cDNA clones that code for portions of the two minor chicken vitellogenin (VTG) genes (VTGI and VTGIII) is reported. These clones represent unique sequences that are expressed exclusively in the livers of estrogenized birds. In the liver of the egg-laying hen, the levels of RNAs encoding VTGI, VTGII, and VTGIII are approximately 11,000, 30,000, and 3,000 molecules per cell, respectively. We have used the newly isolated clones, as well as the yolk protein cDNAs previously available [VTGII, apolipoprotein II (apoVLDLII), and apolipoprotein B], as probes to examine several aspects of the regulation of these genes by estradiol. First, we demonstrate that the capacity of each gene to respond to estradiol is acquired between 8 and 13 days in ovo. The response of four of these genes to estradiol is diminished during late fetal development, but the responsiveness is recovered within a week after hatching. Second, we demonstrate that these genes display distinct kinetic response profiles following the addition of estradiol. Third, as has been described previously for the VTGII and apoVLDLII genes, we demonstrate that a single injection of estradiol effects a long-term reprogramming event (hepatic memory) that allows a faster onset of the rapid accumulation of both VTGI and VTGIII RNAs following a subsequent rechallenge by estradiol. Collectively, these three sets of data suggest molecular parameters that may contribute to both the coordinate and noncoordinate regulation of this set of genes by estradiol.