Effects of ethyleneglycol chain length of dodecyl polyethyleneglycol monoether on the crystallization of bovine heart cytochrome c oxidase.
Effects of ethyleneglycol chain length of dodecyl polyethyleneglycol monoether on the crystallization of bovine heart cytochrome c oxidase.
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十二烷基聚乙二醇单醚乙二醇链长对牛心细胞色素c氧化酶结晶的影响
DOI：
10.1016/s0022-2836(05)80106-8
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发表时间：
1995
影响因子：
5.6
通讯作者：
T. Tsukihara
中科院分区：
文献类型：
--
作者：
K. Shinzawa;H. Ueda;S. Yoshikawa;H. Aoyama;E. Yamashita;T. Tsukihara
从牛心脏细胞色素c氧化酶中分离并用十二烷基八乙二醇单醚CH 3（CH 2）11 O（CH 2CH 2 O）8H增溶得到衍射X射线分辨率高达5 A的四氢呋喃晶体。在10 A/cm、7 A/cm和6 A/cm分辨率下，从不同天然晶体获得的数据集之间的观察到的结构因子的比较分别给出了0.92、0.84和0.57的相关系数。晶体空间群为I41或I43，晶胞参数为a = B = 253 A，c = 507 A。四氢呋喃晶体的完美性和稳定性明显高于用Brij-35，CH 3（CH 2）11 O（CH 2CH 2 O）23 H稳定的蛋白质的六方晶体的完美性和稳定性（其细节在别处报道）。乙二醇链长对结晶的影响的检查显示，只有十二烷基聚乙二醇单醚与八个和七个单元是适当的生产这种类型的晶体，表明膜蛋白结晶的洗涤剂的最佳尺寸。
Tetragonal crystals that diffracted X-rays up to 5 Å resolution were obtained from bovine heart cytochrome c oxidase isolated and solubilized with dodecyl octaethyleneglycol monoether, CH3(CH2)11O(CH2CH2O)8H. Comparison of observed structure factors between data sets each obtained from a different native crystal gave correlation coefficients of 0.92, 0.84 and 0.57 at 10 Å, 7 Å and 6 Å resolution, respectively. The space group and the cell dimensions of the crystal are I41or I43and a = b = 253 Å, c = 507 Å, respectively. The perfection and stability of the tetragonal crystals are significantly higher than those of the hexagonal crystals of the protein stabilized with Brij-35, CH3(CH2)11O(CH2CH2O)23H (whose details are reported elsewhere). Examination of the effect of ethyleneglycol chain length on the crystallization revealed that only dodecyl polyethyleneglycol monoethers with eight and seven units were appropriate for producing this type of crystal, indicating an optimum size of the detergent for crystallization of the membrane protein.