Prescribed nanoparticle cluster architectures and low-dimensional arrays built using octahedral DNA origami frames.
Prescribed nanoparticle cluster architectures and low-dimensional arrays built using octahedral DNA origami frames.
复制标题
使用八面体DNA折纸框架构建的规定纳米颗粒群结构和低维数组。
DOI：
10.1038/nnano.2015.105
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发表时间：
2015-07
影响因子：
38.3
通讯作者：
Gang O
中科院分区：
文献类型：
--
作者：
Tian Y;Wang T;Liu W;Xin HL;Li H;Ke Y;Shih WM;Gang O
可靠地创建三维（3D）中尺度簇，其中纳米粒子在空间上排列在预定的位置，是迈向任意设计的自组装结构的第一步。这些工程化的簇将是分子的中尺度类似物，并且因此由于集体效应而提供定制的纳米颗粒性质。然而，建立一个灵活且广泛适用的平台来制造纳米颗粒架构以及探测其3D非周期性组织是具有挑战性的。在这里，我们报告了一种新的战略组装三维纳米粒子簇：设计一个分子框架与编码的顶点粒子的位置。通过将特定的粒子类型定位在这种框架的顶点，DNA折纸八面体，我们制造了各种对称性和粒子组成的集群。我们应用Cryo-EM方法来揭示DNA框架结构，并揭示纳米颗粒以规定的方式空间配位。采用所展示的组装策略，我们已经创建了具有不同手性活性的纳米簇，其基于特定编码的中心对称DNA框架和相同的纳米颗粒集。我们还表明，八面体与特别选定的顶点可以作为可定制的粒子间连接器与一定的几何形状的粒子间连接，因此，允许组装的1D或2D阵列与设计的粒子排列。
Reliably creating three-dimensional (3D) mesoscale clusters, in which nanoparticles are spatially arranged in pre-determined positions, is the first step towards arbitrarily designed self-assembled architectures. These engineered clusters would be the mesoscale analogs of molecules and would thus offer tailored nanoparticles properties due to the collective effects. However, establishing a flexible and broadly applicable platform for the fabrication of nanoparticles architectures, as well as probing their 3D non-periodic organizations, is challenging. Here we report a novel strategy for assembling 3D nanoparticle clusters: designing a molecular frame with encoded vertices for particles placement. By positioned specific particles types at the vertices of such frame, a DNA origami octahedron, we fabricated clusters of various symmetries and particles composition. We applied the Cryo-EM methods to uncover the DNA frame structure, and to reveal that nanoparticles are spatially coordinated in the prescribed manner. Employing the demonstrated assembly strategy, we have created nanoclusters with different chiroptical activities based on the specifically encoded center-symmetrical DNA frame and the same set of nanoparticles. We also show that octahedra with particularly selected vertices can serve as tailorable interparticle linker with a certain geometry of interparticle connections, thus, allowing for assembly of 1D or 2D arrays with designed particle arrangements.