Common marmoset (Callithrix jacchus) as a primate model for behavioral neuroscience studies
Common marmoset (Callithrix jacchus) as a primate model for behavioral neuroscience studies
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DOI：
10.1016/j.jneumeth.2017.04.004
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发表时间：
2017-06-01
影响因子：
3
通讯作者：
Prasad, Abhishek
中科院分区：
文献类型：
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作者：
Prins, Noeline W.;Pohlmeyer, Eric A.;Prasad, Abhishek
背景：普通狨猴（Callithrix jacchus）被认为是啮齿类动物和大型灵长类动物之间的合适桥梁。它们已被用于多种类型的研究，包括听觉、声音、视觉、药理学和遗传学研究。然而，狨猴还没有被广泛用于行为研究。新方法：在这里，我们展示了训练 12 只成年狨猴进行行为神经科学研究的数据。我们讨论饲养、食物偏好、处理、实验室环境的适应和神经外科技术。在本文中，我们还提出了一个定制的“勺子”和一个适合训练这些动物的猴子椅。结果：这些动物接受了三项任务的训练：4 个目标中心向外的到达任务、涉及控制机器人动作的到达任务和触摸屏任务。所有动物都在 1-2 周内学会了从中心向外的伸手任务，而学习控制机器人动作的伸手任务需要几个月的行为训练，猴子学会将机器人动作与食物奖励联系起来。与现有方法的比较：我们建议将狨猴作为行为神经科学研究的新模型，作为大型灵长类动物模型的替代品。这是由于易于操作、快速繁殖、可用的神经解剖学、与大型灵长类动物和人类相似的感觉运动系统，以及与大型灵长类动物相比，可以在不同皮质位置相对更容易地植入微电极阵列的无脑大脑。结论：所有动物都能够很好地学习行为任务，我们将狨猴作为简单行为神经科学任务的替代模型。 (C) 2017 Elsevier B.V. 保留所有权利。
Background: The common marmoset (Callithrix jacchus) has been proposed as a suitable bridge between rodents and larger primates. They have been used in several types of research including auditory, vocal, visual, pharmacological and genetics studies. However, marmosets have not been used as much for behavioral studies.New method: Here we present data from training 12 adult marmosets for behavioral neuroscience studies. We discuss the husbandry, food preferences, handling, acclimation to laboratory environments and neurosurgical techniques. In this paper, we also present a custom built "scoop" and a monkey chair suitable for training of these animals.Results: The animals were trained for three tasks: 4 target center-out reaching task, reaching tasks that involved controlling robot actions, and touch screen task. All animals learned the center-out reaching task within 1-2 weeks whereas learning reaching tasks controlling robot actions task took several months of behavioral training where the monkeys learned to associate robot actions with food rewards. Comparison to existing method: We propose the marmoset as a novel model for behavioral neuroscience research as an alternate for larger primate models. This is due to the ease of handling, quick reproduction, available neuroanatomy, sensorimotor system similar to larger primates and humans, and a lissencephalic brain that can enable implantation of microelectrode arrays relatively easier at various cortical locations compared to larger primates.Conclusion: All animals were able to learn behavioral tasks well and we present the marmosets as an alternate model for simple behavioral neuroscience tasks. (C) 2017 Elsevier B.V. All rights reserved.